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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-4-16
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pubmed:abstractText |
PNH is an acquired hematopoietic stem cell disorder leading to a partial or absolute deficiency of all glycophosphatidyl-inositol (GPI)-linked proteins. The classical approach to diagnosis of PNH by cytometry involves the loss of at least two GPI-linked antigens on RBCs and neutrophils. While flow assays are more sensitive and specific than complement-mediated lysis or the Hams test, they suffer from several drawbacks. Bacterial aerolysin binds to the GPI moiety of cell surface GPI-linked molecules and causes lysis of normal but not GPI-deficient PNH cells. FLAER is an Alexa488-labeled inactive variant of aerolysin that does not cause lysis of cells. Our goals were to develop a FLAER-based assay to diagnose and monitor patients with PNH and to improve detection of minor populations of PNH clones in other hematologic disorders.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD59,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/CD33 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CD59 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/PTPRC protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Reagent Kits, Diagnostic,
http://linkedlifedata.com/resource/pubmed/chemical/proaerolysin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1552-4949
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2007 Clinical Cytometry Society.
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-77
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pubmed:dateRevised |
2009-12-11
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pubmed:meshHeading |
pubmed-meshheading:17285629-Antigens, CD,
pubmed-meshheading:17285629-Antigens, CD14,
pubmed-meshheading:17285629-Antigens, CD45,
pubmed-meshheading:17285629-Antigens, CD59,
pubmed-meshheading:17285629-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:17285629-Bacterial Toxins,
pubmed-meshheading:17285629-Drug Stability,
pubmed-meshheading:17285629-Erythrocytes,
pubmed-meshheading:17285629-Flow Cytometry,
pubmed-meshheading:17285629-Fluorescent Dyes,
pubmed-meshheading:17285629-Hematologic Diseases,
pubmed-meshheading:17285629-Hemoglobinuria, Paroxysmal,
pubmed-meshheading:17285629-Humans,
pubmed-meshheading:17285629-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:17285629-Reagent Kits, Diagnostic,
pubmed-meshheading:17285629-Sensitivity and Specificity
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pubmed:year |
2007
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pubmed:articleTitle |
Diagnosing PNH with FLAER and multiparameter flow cytometry.
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pubmed:affiliation |
Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada. rob.southerland@utoronto.ca
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pubmed:publicationType |
Journal Article,
Comparative Study,
Evaluation Studies
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