|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
26
|
|
pubmed:dateCreated |
2007-2-6
|
|
pubmed:abstractText |
We demonstrate apertureless near-field microscopy of single molecules at sub-10 nm resolution. With a novel phase filter, near-field images of single organic fluorophores were obtained with approximately sixfold improvement in the signal-to-noise ratio. The improvement allowed pairs of molecules separated by approximately 15 nm to be reliably and repeatedly resolved, thus demonstrating the first true Rayleigh resolution test for near-field images of single molecules. The potential of this technique for biological applications was demonstrated with an experiment that measured the helical rise of A-form DNA.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0031-9007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
31
|
|
pubmed:volume |
97
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
260801
|
|
pubmed:meshHeading |
pubmed-meshheading:17280412-Algorithms,
pubmed-meshheading:17280412-Carbocyanines,
pubmed-meshheading:17280412-DNA, A-Form,
pubmed-meshheading:17280412-Fluorescent Dyes,
pubmed-meshheading:17280412-Microscopy, Atomic Force,
pubmed-meshheading:17280412-Microscopy, Confocal,
pubmed-meshheading:17280412-Microscopy, Fluorescence,
pubmed-meshheading:17280412-Nucleic Acid Conformation,
pubmed-meshheading:17280412-Silicon
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Fluorescence near-field microscopy of DNA at sub-10 nm resolution.
|
|
pubmed:affiliation |
Applied Physics and Physics, California Institute of Technology, Pasadena, California 91125, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
