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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-2-21
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pubmed:abstractText |
Our current knowledge of the general factor requirement in transcription by the three mammalian RNA polymerases is based on a small number of model promoters. Here, we present a comprehensive chromatin immunoprecipitation (ChIP)-on-chip analysis for 28 transcription factors on a large set of known and novel TATA-binding protein (TBP)-binding sites experimentally identified via ChIP cloning. A large fraction of identified TBP-binding sites is located in introns or lacks a gene/mRNA annotation and is found to direct transcription. Integrated analysis of the ChIP-on-chip data and functional studies revealed that TAF12 hitherto regarded as RNA polymerase II (RNAP II)-specific was found to be also involved in RNAP I transcription. Distinct profiles for general transcription factors and TAF-containing complexes were uncovered for RNAP II promoters located in CpG and non-CpG islands suggesting distinct transcription initiation pathways. Our study broadens the spectrum of general transcription factor function and uncovers a plethora of novel, functional TBP-binding sites in the human genome.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-10202152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11125145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11237001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11250901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11395415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11799067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11893486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-11988577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-12191469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-12353038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-12381659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-12504019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-14980218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-14992726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15006352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15145350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15525513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15539566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15680324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15735639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15790807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-15988478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-16126390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-16413481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-16625203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-7842006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-8663456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-9064287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-9254723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-9674425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17268553-9885574
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
944-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17268553-Binding Sites,
pubmed-meshheading:17268553-Chromatin Immunoprecipitation,
pubmed-meshheading:17268553-CpG Islands,
pubmed-meshheading:17268553-Gene Expression Regulation,
pubmed-meshheading:17268553-Genome, Human,
pubmed-meshheading:17268553-Humans,
pubmed-meshheading:17268553-Microarray Analysis,
pubmed-meshheading:17268553-Principal Component Analysis,
pubmed-meshheading:17268553-RNA Polymerase I,
pubmed-meshheading:17268553-RNA Polymerase II,
pubmed-meshheading:17268553-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17268553-TATA-Binding Protein Associated Factors,
pubmed-meshheading:17268553-TATA-Box Binding Protein,
pubmed-meshheading:17268553-Transcription Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Identification of novel functional TBP-binding sites and general factor repertoires.
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pubmed:affiliation |
Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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