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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-12-7
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pubmed:abstractText |
A gene encoding a temperature-sensitive mutant (MPP154A) of the 30-kilodalton movement protein (MP) of tobacco mosaic virus (TMV) was transformed into Nicotiana tabacum cv Xanthi. Transgenic plants expressing the MPP154A gene complemented local and systemic movement of an MP-defective mutant of TMV (U3/12MPfs) at the permissive temperature of 24 degrees C but not at 32 degrees C, the nonpermissive temperature. A microinjection procedure was used to investigate the effects of the modified TMV MP on plasmodesmatal size-exclusion limits. Movement of fluorescein isothiocyanate-labeled dextran (F-dextran), with an average molecular mass of 9.4 kilodaltons, was detected between leaf mesophyll cells of the transgenic plants at 24 degrees C; however, no movement of either 3.9-kilodalton or 9.4-kilodalton F-dextrans was detected when the transgenic plants were held for 6 hours (or longer) at 32 degrees C. When these plants were shifted back to 24 degrees C for 6 hours, cell-to-cell movement of the F-dextrans was again observed. Accumulation of MPP154A was not affected by the temperature regime, nor was the subcellular distribution of the MP altered. These results are consistent with a change in the protein conformation of MPP154A at the nonpermissive temperature, which gives rise to a protein that fails to modify the molecular size-exclusion limits of plasmodesmata to the same extent as wild-type MP. Surprisingly, at 32 degrees C, movement of the F-dextrans was inhibited in transgenic plants expressing the wild-type MP gene; however, the inhibition was transient and was no longer detected after 48 hours at this elevated temperature. This transient inhibition of plasmodesmatal function was alleviated with Sirofluor, an inhibitor of callose ([1----3]-beta-D-glucan) synthesis. This result provides experimental evidence that callose deposition is involved in regulating the molecular size-exclusion limit of plasmodesmata in plants. Sirofluor had no effect on the inhibition of F-dextran movement at 32 degrees C in plants expressing the MPP154A gene, indicating that callose formation was not responsible for the failure of the temperature-sensitive mutant protein to alter the size-exclusion limit of plasmodesmata.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-10976202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-1264250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-16453793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-16656012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-16665479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-17757862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-17794341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-1984651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-1990065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-1994570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-2238467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-2302736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-2333282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-354496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-3776102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-5781865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-7197900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-726262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-888358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1726784-936472
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
593-604
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1726784-Benzenesulfonates,
pubmed-meshheading:1726784-Biological Transport,
pubmed-meshheading:1726784-Cell Membrane,
pubmed-meshheading:1726784-Cell Wall,
pubmed-meshheading:1726784-Dextrans,
pubmed-meshheading:1726784-Fluorescent Dyes,
pubmed-meshheading:1726784-Glucans,
pubmed-meshheading:1726784-Hot Temperature,
pubmed-meshheading:1726784-Mutation,
pubmed-meshheading:1726784-Organelles,
pubmed-meshheading:1726784-Plant Viral Movement Proteins,
pubmed-meshheading:1726784-Plants, Genetically Modified,
pubmed-meshheading:1726784-Plants, Toxic,
pubmed-meshheading:1726784-Tobacco,
pubmed-meshheading:1726784-Viral Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein.
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pubmed:affiliation |
Botany Department, University of California, Davis 95616-8537.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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