Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-12-1
|
| pubmed:abstractText |
We analysed the glycolipids of mouse thymocytes before and after Concanavalin A (Con A) or recombinant interleukin-2 (rIL-2) stimulation by TLC-immunostaining with carbohydrate-specific antiglycolipid antibodies. The thymocytes were cultured in serum-free medium in the presence of 500 ng ml-1 Con A, 10 U ml-1 rIL-2 or Con A plus rIL-2 for 6, 12, 24, 48, and 72 h, and were found to start proliferating 24 h after cultivation in the presence of Con A or Con A plus rIL-2, the maximum levels being reached at 72 h and 48 h, respectively, in a thymidine uptake experiment. The concentrations of II3Neu-Gg4Cer, Gg4Cer and IV3GalNAc alpha-Gb4Cer after 48 h Con A stimulation were found to be at almost the original levels. Conversely, II3Neu-Gg3Cer, which was not detected in the thymocytes at the start, began to appear after 48 h stimulation with Con A and Con A plus rIL-2, and IV3Neu-Gg5Cer in the cells 48 h after stimulation with Con A and Con A plus rIL-2 has increased to 41 and 44 times higher than in the original cells, respectively, as judged on TLC-immunostaining with monoclonal antibody YHD-06, which detects the GalNAc beta 1-4(NeuAc or NeuGc alpha 2-3)Gal beta-structure. These results indicate that the increased synthesis of both gangliosides, in other words, the activation of N-acetylgalactosaminyltransferase, is associated with the mitogen-induced proliferation.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gangliosides,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0282-0080
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
354-60
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1726779-Animals,
pubmed-meshheading:1726779-Antibodies, Monoclonal,
pubmed-meshheading:1726779-Antibody Specificity,
pubmed-meshheading:1726779-Carbohydrate Conformation,
pubmed-meshheading:1726779-Carbohydrate Sequence,
pubmed-meshheading:1726779-Cell Division,
pubmed-meshheading:1726779-Cells, Cultured,
pubmed-meshheading:1726779-Concanavalin A,
pubmed-meshheading:1726779-Epitopes,
pubmed-meshheading:1726779-Female,
pubmed-meshheading:1726779-Flow Cytometry,
pubmed-meshheading:1726779-Gangliosides,
pubmed-meshheading:1726779-Immunoenzyme Techniques,
pubmed-meshheading:1726779-Interleukin-2,
pubmed-meshheading:1726779-Mice,
pubmed-meshheading:1726779-Mice, Inbred BALB C,
pubmed-meshheading:1726779-Molecular Sequence Data,
pubmed-meshheading:1726779-Molecular Structure,
pubmed-meshheading:1726779-Recombinant Proteins,
pubmed-meshheading:1726779-Thymus Gland
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Concanavalin A-stimulated expression of gangliosides with GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta structure in murine thymocytes.
|
| pubmed:affiliation |
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|