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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-4-3
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pubmed:abstractText |
Surveillance of group A streptococcal (GAS) infections was undertaken as a major component of the European Commission-funded project on severe GAS disease in Europe (strep-EURO). One aim of strep-EURO was to improve the quality of GAS characterization by standardization of methods. An external quality assurance study (EQA) was therefore carried out to evaluate current global performance. Eleven strep-EURO and seven other streptococcal reference centers received a panel of 20 coded GAS isolates for typing. Conventional phenotypic typing (based on cell surface T and M protein antigens and opacity factor [OF] production) and molecular methods (emm gene typing) were used either as single or combined approaches to GAS typing. T typing was performed by 16 centers; 12 centers found one or more of the 20 strains nontypeable (typeability, 89%), and 11 centers reported at least one incorrect result (concordance, 93%). The 10 centers that tested for OF production achieved 96% concordance. Limited availability of antisera resulted in poor typeability values from the four centers that performed phenotypic M typing (41%), three of which also performed anti-OF typing (typeability, 63%); however, concordance was high for both M (100%) and anti-OF (94%) typing. In contrast, the 15 centers that performed emm gene sequencing achieved excellent typeability (97%) and concordance (98%), although comparison of the performance between centers yielded typeability rates from 65 to 100% and concordance values from 83 to 100%. With the rapid expansion and use of molecular genotypic methods to characterize GAS, continuation of EQA is essential in order to achieve international standardization and comparison of type distributions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-10221877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-10832648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-10885988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-11254602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-11731942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-12115092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-12748273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-14461914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-14532198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-15189292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-15304473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-15524268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-16434707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-1784271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-8039659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-8170398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-8815115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17267628-9316938
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0095-1137
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pubmed:author |
pubmed-author:BeallBernardB,
pubmed-author:EfstratiouAndroullaA,
pubmed-author:EkelundKimK,
pubmed-author:Henriques-NormarkBirgittaB,
pubmed-author:International Streptococcus Reference Laboratories,
pubmed-author:JasirAftabA,
pubmed-author:JohnsonDwightD,
pubmed-author:KaplanEdwardE,
pubmed-author:LovgrenMargueriteM,
pubmed-author:NealShonaS,
pubmed-author:ReinertRalf ReneRR,
pubmed-author:Strep-EURO Study Group
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1175-9
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pubmed:dateRevised |
2010-9-16
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pubmed:meshHeading |
pubmed-meshheading:17267628-Antigens, Bacterial,
pubmed-meshheading:17267628-Bacterial Outer Membrane Proteins,
pubmed-meshheading:17267628-Bacterial Typing Techniques,
pubmed-meshheading:17267628-Bacteriological Techniques,
pubmed-meshheading:17267628-Carrier Proteins,
pubmed-meshheading:17267628-Europe,
pubmed-meshheading:17267628-Genotype,
pubmed-meshheading:17267628-Humans,
pubmed-meshheading:17267628-Peptide Hydrolases,
pubmed-meshheading:17267628-Quality Control,
pubmed-meshheading:17267628-Sequence Analysis, DNA,
pubmed-meshheading:17267628-Serotyping,
pubmed-meshheading:17267628-Streptococcal Infections,
pubmed-meshheading:17267628-Streptococcus pyogenes
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pubmed:year |
2007
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pubmed:articleTitle |
International quality assurance study for characterization of Streptococcus pyogenes.
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pubmed:affiliation |
Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5HT, United Kingdom. shona.neal@hpa.org.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Multicenter Study
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