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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1992-8-19
|
| pubmed:abstractText |
Rabbit polyclonal antibody (Poly-1) and mouse monoclonal antibodies (mAbs) TO-11, TO-14 and TO-M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly-1 and the 3 mAbs were screened for their reactivity with whole cells oral and nonoral black-pigmented bacterial species by enzyme-linked immunosorbent assay (ELISA) and the binding experiment using [125I]Poly-1 and [125I]mAbs. ELISA revealed that Poly-1 definitely reacted with whole cells of all the 11 strains of P. gingivalis tested. However, 8 of 11 P. gingivalis strains reacted with mAbs TO-11, TO-14 and TO-M1. These results were confirmed by the specific binding of radiolabelled Poly-1 and mAb TO-11 to the 8 strains. The M(r) of the fimbrial subunit protein (fimbrilin) isolated and purified from P. gingivalis strains 381, BH18/10, HW24D-1, 6/26 and OMZ 314 was 41 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It was found by immunoblotting that mAbs TO-11 and TO-14/TO-M1 recognized different epitopes of fimbrial protein from P. gingivalis strains. Immunoelectron micrographs of whole cells and the purified fimbriae of P. gingivalis strains visualized similar serotype-specific antibody bindings to the fimbriae. These results indicate that 11 strains of P. gingivalis could be divided into at least 2 separate groups based on the immunochemical specificities of the fimbriae.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0902-0055
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
332-40
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1726543-Animals,
pubmed-meshheading:1726543-Bacterial Adhesion,
pubmed-meshheading:1726543-Bacterial Proteins,
pubmed-meshheading:1726543-Bacteroides,
pubmed-meshheading:1726543-Blotting, Western,
pubmed-meshheading:1726543-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1726543-Epitopes,
pubmed-meshheading:1726543-Fimbriae, Bacterial,
pubmed-meshheading:1726543-Male,
pubmed-meshheading:1726543-Mice,
pubmed-meshheading:1726543-Mice, Inbred BALB C,
pubmed-meshheading:1726543-Periodontal Diseases,
pubmed-meshheading:1726543-Porphyromonas gingivalis,
pubmed-meshheading:1726543-Species Specificity
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species.
|
| pubmed:affiliation |
Osaka University Faculty of Dentistry, Japan.
|
| pubmed:publicationType |
Journal Article
|