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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1992-7-24
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pubmed:abstractText |
The antibody response in cats to feline immunodeficiency virus (FIV) reverse transcriptase (RT) was followed for 3 years. Eight of the nine cats used in this study produced reverse transcriptase-inhibiting (RTI) antibodies. Relative inhibitory means of 2.9%, 18.4%, 33%, and 47% were found 6, 12, 24, and 36 months, respectively, after infection with FIV. The enzyme activity was suppressed by greater than or equal to 78% with the use of 100 micrograms of FIV-associated IgG. The RTI antibodies were FIV-specific, as they did not inhibit other mammalian retroviral polymerases, including feline leukemia virus RT. An RT-inhibition assay with sera in the presence of protein A and immunoblot analysis showed that antibody binding to FIV RT protein p62 is independent of antibody ability to block enzyme activity. Viral RT released by detergent-treated virus was stable for more than 6 weeks at 4 degrees C, whereas its activity was reduced by 50% after 2 weeks at 37 degrees C. Because significant concentrations of RTI antibodies are detected only at 1 to 2 years after infection, they can be used to determine the approximate time of virus infection and as a marker for disease progression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Reverse Transcriptase Inhibitors
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pubmed:status |
MEDLINE
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pubmed:issn |
0882-8245
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-35
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1726399-Animals,
pubmed-meshheading:1726399-Antibodies, Viral,
pubmed-meshheading:1726399-CD4-CD8 Ratio,
pubmed-meshheading:1726399-Cats,
pubmed-meshheading:1726399-Feline Acquired Immunodeficiency Syndrome,
pubmed-meshheading:1726399-Immunodeficiency Virus, Feline,
pubmed-meshheading:1726399-Leukemia Virus, Feline,
pubmed-meshheading:1726399-Moloney murine leukemia virus,
pubmed-meshheading:1726399-RNA-Directed DNA Polymerase,
pubmed-meshheading:1726399-Retroviridae,
pubmed-meshheading:1726399-Retroviridae Proteins,
pubmed-meshheading:1726399-Reverse Transcriptase Inhibitors,
pubmed-meshheading:1726399-Specific Pathogen-Free Organisms
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pubmed:year |
1991
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pubmed:articleTitle |
Antibody response to reverse transcriptase in cats infected with feline immunodeficiency virus.
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pubmed:affiliation |
Department of Veterinary Microbiology, Immunology and Parasitology, New York State College of Veterinary Medicine, Cornell University, Ithaca.
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pubmed:publicationType |
Journal Article,
Comparative Study
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