Linked Life Data

17260136

Source:http://linkedlifedata.com/resource/pubmed/id/17260136

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-2-20
pubmed:abstractText
Application of matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) to small-molecule detection is often limited, because of high matrix background signals in the low-mass region. We report here an approach in which a mixture of two conventional MALDI matrices with different proton affinity was used to suppress the formation of matrix clusters and fragments. Specifically, when acidic alpha-cyano-4-hydroxycinnamic acid (CHCA) and basic 9-aminoacridine (9-AA) were used as the binary matrix, fewer background matrix peaks were observed in both positive and negative-mode detection of small molecules. In addition, the presence of CHCA substantially reduced the laser fluence needed for analyte desorption and ionization; thus better signal-to-background ratios were observed for negatively charged inositol phosphates in complex plant extracts.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Mar
pubmed:issn
1618-2642
pubmed:author
pubmed:issnType
Print
pubmed:volume
387
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1939-44
pubmed:year
2007
pubmed:articleTitle
A binary matrix for background suppression in MALDI-MS of small molecules.
pubmed:affiliation
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
pubmed:publicationType
Journal Article