Linked Life Data

17258828

Source:http://linkedlifedata.com/resource/pubmed/id/17258828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-3-23
pubmed:abstractText
The application of quantitative real-time PCR has been proposed for the quantification of toxic genotypes of cyanobacteria. We have compared the Taq Nuclease Assay (TNA) in quantifying the toxic cyanobacteria Microcystis sp. via the intergenic spacer region of the phycocyanin operon (PC) and mcyB indicative of the production of the toxic heptapeptide microcystin between three research groups employing three instruments (ABI7300, GeneAmp5700, ABI7500). The estimates of mcyB genotypes were compared using (i) DNA of a mcyB containing strain and a non-mcyB containing strain supplied in different mixtures across a low range of variation (0-10% of mcyB) and across a high range of variation (20-100%), and (ii) DNA from field samples containing Microcystis sp. For all three instruments highly significant linear regression curves between the proportion of the mcyB containing strain and the percentage of mcyB genotypes both within the low range and within the high range of mcyB variation were obtained. The regression curves derived from the three instruments differed in slope and within the high range of mcyB variation mcyB proportions were either underestimated (0-50%) or overestimated (0-72%). For field samples cell numbers estimated via both TNAs as well as mcyB proportions showed significant linear relationships between the instruments. For all instruments a linear relationship between the cell numbers estimated as PC genotypes and the cell numbers estimated as mcyB genotypes was observed. The proportions of mcyB varied from 2 to 28% and did not differ between the instruments. It is concluded that the TNA is able to provide quantitative estimates on mcyB genotype numbers that are reproducible between research groups and is useful to follow variation in mcyB genotype proportion occurring within weeks to months.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-11055948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-11411709, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-11984633, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-12392529, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-12570996, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-12700152, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-14602633, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-14660378, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-15984800, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-16461672, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-16470363, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-16599997, http://linkedlifedata.com/resource/pubmed/commentcorrection/17258828-9427407
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0167-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
122-8
pubmed:dateRevised
2011-7-25
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Interlaboratory comparison of Taq Nuclease Assays for the quantification of the toxic cyanobacteria Microcystis sp.
pubmed:affiliation
Institute for Limnology, Austrian Academy of Sciences, 5310 Mondsee, Austria.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't