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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-2-13
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pubmed:abstractText |
To investigate the effect of Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) on human cancer cells, we sought to identify and analyze potential target genes that were differentially expressed in the presence and absence of LMP1. Our cDNA microarray analysis revealed that expression of early growth response gene-1 (Egr-1) was increased by LMP1 expression in MCF7 and Jurkat cells. An NFkappaB inhibitor (SN50) antagonized LMP1-induced enhancement of Egr-1 expression, indicating that LMP1 induced Egr-1 via NFkappaB. Furthermore, three lines of evidence indicated that Egr-1 was required for LMP1-induced cancer cell survival. First, Egr-1 expression enhanced the survival of doxorubicin-treated MCF7 cells. Second, inhibition of Egr-1 expression by siRNA (siEgr-1) effectively suppressed LMP-1-induced survival of MCF7 cells. Third, Egr-1 knockdown decreased LMP1-induced expression of Bfl-1. Similar relationships among EBV infection, Egr-1 and drug resistance were also observed in tissues of peripheral T-cell lymphoma-unspecified (PTCL-u) patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
581
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-8
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pubmed:meshHeading |
pubmed-meshheading:17257596-Antiviral Agents,
pubmed-meshheading:17257596-Cell Survival,
pubmed-meshheading:17257596-Drug Resistance, Viral,
pubmed-meshheading:17257596-Early Growth Response Protein 1,
pubmed-meshheading:17257596-Epstein-Barr Virus Infections,
pubmed-meshheading:17257596-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:17257596-Herpesviridae,
pubmed-meshheading:17257596-Humans,
pubmed-meshheading:17257596-Jurkat Cells,
pubmed-meshheading:17257596-NF-kappa B,
pubmed-meshheading:17257596-RNA, Messenger,
pubmed-meshheading:17257596-RNA, Small Interfering,
pubmed-meshheading:17257596-Up-Regulation,
pubmed-meshheading:17257596-Viral Matrix Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Egr-1, a new downstream molecule of Epstein-Barr virus latent membrane protein 1.
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pubmed:affiliation |
Biomedical Research Institute, Samsung Medical Center, 50 Irwon-dong, Kangnam-gu, Seoul 135-710, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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