Linked Life Data

17255522

Source:http://linkedlifedata.com/resource/pubmed/id/17255522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-5-1
pubmed:abstractText
Cardiomyocytes derived from human embryonic stem cells constitute a promising cell source for the regeneration of damaged hearts. The assessment of their in vitro functional properties is mandatory to envisage appropriate cardiac cell-based therapies. In this study, we characterized human embryonic stem cell-derived cardiomyocytes over a 3-month period, using patch-clamp or intracellular recordings to assess their functional maturation and reverse transcriptase-polymerase chain reaction to evaluate the expression of ion channel-encoding subunits. I(to1) and I(K1), the transient outward and inward rectifier potassium currents, were present in cardiomyocytes only, whereas the rapid delayed rectifier potassium current (I(Kr)), pacemaker current (I(f)), and L-type calcium current (I(Ca,L)) could be recorded both in undifferentiated human embryonic stem cells and in cardiomyocytes. Most of the currents underwent developmental maturation in cardiomyocytes, as assessed by modifications in current density (I(to1), I(K1), and I(Ca,L)) and properties (I(f)). Ion-channel mRNAs were always present when the current was recorded. Intracellular recordings in spontaneously beating clusters of cardiomyocytes revealed changes in action potential parameters and in response to pharmacological tools according to time of differentiation. In summary, human embryonic stem cell-derived cardiomyocytes mature over time during in vitro differentiation, approaching an adult phenotype. Disclosure of potential conflicts of interest is found at the end of this article.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1136-44
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:17255522-Animals, pubmed-meshheading:17255522-Calcium Channels, L-Type, pubmed-meshheading:17255522-Cell Differentiation, pubmed-meshheading:17255522-Cells, Cultured, pubmed-meshheading:17255522-Cyclic Nucleotide-Gated Cation Channels, pubmed-meshheading:17255522-Diastole, pubmed-meshheading:17255522-Electrophysiology, pubmed-meshheading:17255522-Embryonic Stem Cells, pubmed-meshheading:17255522-Gene Expression Regulation, pubmed-meshheading:17255522-Humans, pubmed-meshheading:17255522-Mice, pubmed-meshheading:17255522-Models, Biological, pubmed-meshheading:17255522-Myocytes, Cardiac, pubmed-meshheading:17255522-Potassium Channels, pubmed-meshheading:17255522-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:17255522-RNA, Messenger, pubmed-meshheading:17255522-Time Factors
pubmed:year
2007
pubmed:articleTitle
Developmental changes in cardiomyocytes differentiated from human embryonic stem cells: a molecular and electrophysiological approach.
pubmed:affiliation
Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata, University of Firenze, Firenze, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't