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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5816
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pubmed:dateCreated |
2007-3-2
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pubmed:abstractText |
How do integral membrane proteins evolve in size and complexity? Using the small multidrug-resistance protein EmrE from Escherichia coli as a model, we experimentally demonstrated that the evolution of membrane proteins composed of two homologous but oppositely oriented domains can occur in a small number of steps: An original dual-topology protein evolves, through a gene-duplication event, to a heterodimer formed by two oppositely oriented monomers. This simple evolutionary pathway can explain the frequent occurrence of membrane proteins with an internal pseudo-two-fold symmetry axis in the plane of the membrane.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
2
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pubmed:volume |
315
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1282-4
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:17255477-Amino Acid Sequence,
pubmed-meshheading:17255477-Antiporters,
pubmed-meshheading:17255477-Cell Membrane,
pubmed-meshheading:17255477-Dimerization,
pubmed-meshheading:17255477-Directed Molecular Evolution,
pubmed-meshheading:17255477-Drug Resistance, Bacterial,
pubmed-meshheading:17255477-Escherichia coli,
pubmed-meshheading:17255477-Escherichia coli Proteins,
pubmed-meshheading:17255477-Ethidium,
pubmed-meshheading:17255477-Evolution, Molecular,
pubmed-meshheading:17255477-Gene Duplication,
pubmed-meshheading:17255477-Membrane Transport Proteins,
pubmed-meshheading:17255477-Molecular Sequence Data,
pubmed-meshheading:17255477-Mutation,
pubmed-meshheading:17255477-Protein Folding,
pubmed-meshheading:17255477-Protein Structure, Secondary,
pubmed-meshheading:17255477-Protein Structure, Tertiary,
pubmed-meshheading:17255477-Protein Subunits,
pubmed-meshheading:17255477-Recombinant Fusion Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Emulating membrane protein evolution by rational design.
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pubmed:affiliation |
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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