Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-5-14
pubmed:abstractText
Extracellular acidification contributes to pain by activating or modulating nociceptor activity. To evaluate acidic signaling from the colon, we characterized acid-elicited currents in thoracolumbar (TL) and lumbosacral (LS) dorsal root ganglion (DRG) neurons identified by content of a fluorescent dye (DiI) previously injected into the colon wall. In 13% of unidentified LS DRG neurons (not labeled with DiI) and 69% of LS colon neurons labeled with DiI, protons activated a sustained current that was significantly and reversibly attenuated by the transient receptor potential vanilloid receptor 1 (TRPV1) antagonist capsazepine. In contrast, 63% of unidentified LS DRG neurons and 4% of LS colon neurons exhibited transient amiloride-sensitive acid-sensing ion channel (ASIC) currents. The peak current density of acid-elicited currents was significantly reduced in colon sensory neurons from TRPV1-null mice, supporting predominant expression of TRPV1 in LS colon sensory neurons, which was also confirmed immunohistochemically. Similar to LS colon DRG neurons, acid-elicited currents in TL colon DRG neurons were mediated predominantly by TRPV1. However, the pH producing half-activation of responses significantly differed between TL and LS colon DRG neurons. The properties of acid-elicited currents in colon DRG neurons suggest differential contributions of ASICs and TRPV1 to colon sensation and likely nociception.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1768-74
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17251322-Acidosis, pubmed-meshheading:17251322-Amiloride, pubmed-meshheading:17251322-Animals, pubmed-meshheading:17251322-Capsaicin, pubmed-meshheading:17251322-Cell Size, pubmed-meshheading:17251322-Cells, Cultured, pubmed-meshheading:17251322-Colon, pubmed-meshheading:17251322-Evoked Potentials, pubmed-meshheading:17251322-Extracellular Fluid, pubmed-meshheading:17251322-Ganglia, Spinal, pubmed-meshheading:17251322-Hydrogen-Ion Concentration, pubmed-meshheading:17251322-Kinetics, pubmed-meshheading:17251322-Lumbosacral Plexus, pubmed-meshheading:17251322-Male, pubmed-meshheading:17251322-Mechanotransduction, Cellular, pubmed-meshheading:17251322-Mice, pubmed-meshheading:17251322-Mice, Inbred C57BL, pubmed-meshheading:17251322-Mice, Knockout, pubmed-meshheading:17251322-Neurons, Afferent, pubmed-meshheading:17251322-Pain, pubmed-meshheading:17251322-Patch-Clamp Techniques, pubmed-meshheading:17251322-Sodium Channel Blockers, pubmed-meshheading:17251322-TRPV Cation Channels, pubmed-meshheading:17251322-Thoracic Nerves
pubmed:year
2007
pubmed:articleTitle
Mouse colon sensory neurons detect extracellular acidosis via TRPV1.
pubmed:affiliation
Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural