Source:http://linkedlifedata.com/resource/pubmed/id/17251322
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-5-14
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| pubmed:abstractText |
Extracellular acidification contributes to pain by activating or modulating nociceptor activity. To evaluate acidic signaling from the colon, we characterized acid-elicited currents in thoracolumbar (TL) and lumbosacral (LS) dorsal root ganglion (DRG) neurons identified by content of a fluorescent dye (DiI) previously injected into the colon wall. In 13% of unidentified LS DRG neurons (not labeled with DiI) and 69% of LS colon neurons labeled with DiI, protons activated a sustained current that was significantly and reversibly attenuated by the transient receptor potential vanilloid receptor 1 (TRPV1) antagonist capsazepine. In contrast, 63% of unidentified LS DRG neurons and 4% of LS colon neurons exhibited transient amiloride-sensitive acid-sensing ion channel (ASIC) currents. The peak current density of acid-elicited currents was significantly reduced in colon sensory neurons from TRPV1-null mice, supporting predominant expression of TRPV1 in LS colon sensory neurons, which was also confirmed immunohistochemically. Similar to LS colon DRG neurons, acid-elicited currents in TL colon DRG neurons were mediated predominantly by TRPV1. However, the pH producing half-activation of responses significantly differed between TL and LS colon DRG neurons. The properties of acid-elicited currents in colon DRG neurons suggest differential contributions of ASICs and TRPV1 to colon sensation and likely nociception.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Capsaicin,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/capsazepine
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0363-6143
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
292
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
C1768-74
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| pubmed:dateRevised |
2007-12-3
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| pubmed:meshHeading |
pubmed-meshheading:17251322-Acidosis,
pubmed-meshheading:17251322-Amiloride,
pubmed-meshheading:17251322-Animals,
pubmed-meshheading:17251322-Capsaicin,
pubmed-meshheading:17251322-Cell Size,
pubmed-meshheading:17251322-Cells, Cultured,
pubmed-meshheading:17251322-Colon,
pubmed-meshheading:17251322-Evoked Potentials,
pubmed-meshheading:17251322-Extracellular Fluid,
pubmed-meshheading:17251322-Ganglia, Spinal,
pubmed-meshheading:17251322-Hydrogen-Ion Concentration,
pubmed-meshheading:17251322-Kinetics,
pubmed-meshheading:17251322-Lumbosacral Plexus,
pubmed-meshheading:17251322-Male,
pubmed-meshheading:17251322-Mechanotransduction, Cellular,
pubmed-meshheading:17251322-Mice,
pubmed-meshheading:17251322-Mice, Inbred C57BL,
pubmed-meshheading:17251322-Mice, Knockout,
pubmed-meshheading:17251322-Neurons, Afferent,
pubmed-meshheading:17251322-Pain,
pubmed-meshheading:17251322-Patch-Clamp Techniques,
pubmed-meshheading:17251322-Sodium Channel Blockers,
pubmed-meshheading:17251322-TRPV Cation Channels,
pubmed-meshheading:17251322-Thoracic Nerves
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| pubmed:year |
2007
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| pubmed:articleTitle |
Mouse colon sensory neurons detect extracellular acidosis via TRPV1.
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| pubmed:affiliation |
Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, N.I.H., Extramural
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