Linked Life Data

17250641

Source:http://linkedlifedata.com/resource/pubmed/id/17250641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-1-25
pubmed:abstractText
1. Ischaemia-reperfusion (I/R) injury, one of the main causes of acute renal failure, still needs satisfactory treatment for routine clinical application. Stobadine, a novel synthetic pyridoindole anti-oxidant, has the ability to reduce tissue injury induced by mechanisms involving reactive oxygen species during I/R. The aim of the present study was to determine the effects of stobadine on renal I/R injury. 2. Forty male Wistar rats were randomly divided into four groups as follows: sham, I/R, stobadine treated and I/R + stobadine treated. Stobadine (2 mg/kg, i.v.) was given intravenously to two groups of rats. The stobadine-treated group was treated with stobadine following sham operation before the abdominal wall was closed, whereas the I/R + stobadine group received stobadine at the beginning of reperfusion. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to assess: (i) serum levels of blood urea nitrogen and creatinine; (ii) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR) and glutathione peroxidase (GPx); (iii) renal morphology; and (iv) immunohistochemical staining for P-selectin. 3. Stobadine was able to significantly attenuate the renal dysfunction as a result of renal I/R injury. Ischaemia-reperfusion resulted in a significant increase in serum and kidney MDA levels and a decrease in serum and kidney GSH. Stobadine treatment at the beginning of reperfusion attenuated both the increased MDA levels and decreased GSH secondary to I/R injury. In addition, the decreased G-6PD activity observed after I/R was significantly attenuated by stobadine treatment. Stobadine did not alter 6-PGD activity after I/R. Neither GR nor GPx activity was significantly changed in the I/R alone or the I/R + stobadine groups compared with the sham group. In addition, stobadine decreased the morphological deterioration and high P-selectin immunoreactivity secondary to renal I/R injury. 4. A pyridoindole anti-oxidant, stobadine exerts a renal protective effect in renal I/R injury, which is probably due to its radical-scavenging and anti-oxidant activities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0305-1870
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
210-6
pubmed:meshHeading
pubmed-meshheading:17250641-Animals, pubmed-meshheading:17250641-Blood Urea Nitrogen, pubmed-meshheading:17250641-Carbolines, pubmed-meshheading:17250641-Creatinine, pubmed-meshheading:17250641-Glucosephosphate Dehydrogenase, pubmed-meshheading:17250641-Glutathione, pubmed-meshheading:17250641-Glutathione Peroxidase, pubmed-meshheading:17250641-Glutathione Reductase, pubmed-meshheading:17250641-Immunohistochemistry, pubmed-meshheading:17250641-Injections, Intravenous, pubmed-meshheading:17250641-Kidney, pubmed-meshheading:17250641-Kidney Diseases, pubmed-meshheading:17250641-Male, pubmed-meshheading:17250641-Malondialdehyde, pubmed-meshheading:17250641-Pentose Phosphate Pathway, pubmed-meshheading:17250641-Phosphogluconate Dehydrogenase, pubmed-meshheading:17250641-Rats, pubmed-meshheading:17250641-Rats, Wistar, pubmed-meshheading:17250641-Reperfusion Injury
pubmed:year
2007
pubmed:articleTitle
Stobadine protects rat kidney against ischaemia/reperfusion injury.
pubmed:affiliation
Department of Nephrology, Gazi University Faculty of Medicine, Ankara, Turkey. galip_guz@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't