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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-3-5
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pubmed:abstractText |
We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confidence protein identifications from gel bands too weak for successful analysis using traditional approaches. This has allowed identification of two proteins not previously described as being type III-secreted in rhizobia, NopM and NopD.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1535-3893
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pubmed:author |
pubmed-author:AultJamesJ,
pubmed-author:BellogínRamon ARA,
pubmed-author:EspunyMaría Del RosarioMdel R,
pubmed-author:López-BaenaFrancisco JavierFJ,
pubmed-author:OlleroFrancisco JavierFJ,
pubmed-author:RodriguesJoão AJA,
pubmed-author:Ruiz-SainzJosé EnriqueJE,
pubmed-author:SumptonDaveD,
pubmed-author:Thomas-OatesJaneJ,
pubmed-author:ThomasJerry RJR,
pubmed-author:VinardellJosé MaríaJM
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1029-37
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pubmed:meshHeading |
pubmed-meshheading:17249710-Bacterial Outer Membrane Proteins,
pubmed-meshheading:17249710-Bacterial Proteins,
pubmed-meshheading:17249710-Chromatography, Liquid,
pubmed-meshheading:17249710-Nanotechnology,
pubmed-meshheading:17249710-Sinorhizobium fredii,
pubmed-meshheading:17249710-Soybeans,
pubmed-meshheading:17249710-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:17249710-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:17249710-Tandem Mass Spectrometry
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pubmed:year |
2007
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pubmed:articleTitle |
NopM and NopD are rhizobial nodulation outer proteins: identification using LC-MALDI and LC-ESI with a monolithic capillary column.
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pubmed:affiliation |
Department of Chemistry, University of York, Heslington, York, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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