Linked Life Data

17246322

Source:http://linkedlifedata.com/resource/pubmed/id/17246322

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-28
pubmed:abstractText
A linkage map in tomato has been developed based on isozyme and random cDNA clones derived from mRNA. Interspecific backcross and F(2) populations of Lycopersicon esculentum and L. pennellii were employed in the linkage analysis. Allelic differences in cDNA markers were based on restriction fragment length polymorphisms detected through Southern analysis. A total of 57 unique cDNA clones have been analyzed. The majority of cDNA markers correspond to single loci and are dispersed throughtout the genome. Of those clones that hybridize to two or more loci, most show genetic independence (ie., they are unlinked). The combination of isozyme, cDNA and previously mapped DNA markers total 112 loci. It is estimated that approximately 92% of the genome can be monitored during segregation with these markers. Molecular maps, such as the one being constructed in tomato, may allow genetic and breeding experiments that previously were not possible.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Apr
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
887-98
pubmed:dateRevised
2010-9-15
pubmed:year
1986
pubmed:articleTitle
Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences.
pubmed:affiliation
Department of Horticulture, New Mexico State University, Las Cruces, New Mexico 88003.
pubmed:publicationType
Journal Article