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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1976-2-9
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pubmed:abstractText |
In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was preincubated with the toxin or added to adrenal cells 10 min before CT. In the preincubation studies a molar ratio of Gm1 to toxin of 3:1 was necessary for half-maximal inhibition of steroidogenesis. On the other hand, horse serum anticholeragenoid neutralized the steroidogenic response to cell-bound CT by 50% if it was added to adrenal monolayer cultures 15 min after the toxin. Specific antiserum was able to neutralized 20% of the toxin-induced activity even if it was added to adrenal cultures 2 h after CT. Phase contrast microscopy demonstrated that partial neutralization of the biochemical effect of CT by horse serum anticholeragenoid was accompanied by partial prevention of toxin-induced rounding of adrenal cells. Further studies showed that pretreatment of cultured adrenal cells with a maximal dose of CT increased cyclic adenosine 3'-5'-monophosphate formation in response to a maximal stimulating dose of adrenocorticotropin. This result suggested potentiation of hormonal activation of adenylate cyclase in intact adrenal tumor cells in response to CT.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-1091565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-1147899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-164020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-16557901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-168575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4125267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4206342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4318781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4324714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4365946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4366242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4366919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4367258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4369861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4370199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4372172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4375665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4593242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4707310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-4987011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/172445-5421424
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
768-71
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pubmed:dateRevised |
2010-9-1
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pubmed:meshHeading |
pubmed-meshheading:172445-Adrenal Gland Neoplasms,
pubmed-meshheading:172445-Adrenocorticotropic Hormone,
pubmed-meshheading:172445-Animals,
pubmed-meshheading:172445-Antitoxins,
pubmed-meshheading:172445-Cholera,
pubmed-meshheading:172445-Cyclic AMP,
pubmed-meshheading:172445-Drug Synergism,
pubmed-meshheading:172445-Enterotoxins,
pubmed-meshheading:172445-Gangliosides,
pubmed-meshheading:172445-Horses,
pubmed-meshheading:172445-Neoplasms, Experimental,
pubmed-meshheading:172445-Neutralization Tests
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pubmed:year |
1975
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pubmed:articleTitle |
Interaction of cholera enterotoxin with cultured adrenal tumor cells.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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