Linked Life Data

17241746

Source:http://linkedlifedata.com/resource/pubmed/id/17241746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-3-12
pubmed:abstractText
Hematopoietic prostaglandin D synthase is a key enzyme in synthesis of prostaglandin D. Hematopoietic prostaglandin D synthase is expressed in microglia of the developing mouse brain. This study determined the serial changes and cellular localization of hematopoietic prostaglandin D synthase, and its role in cerebral ischemia/reperfusion injury using C57BL/6 mice (n=84) and bone marrow chimera mice (n=16). The latter mice were selected based on their expression of enhanced green fluorescent protein in bone marrow/blood-derived monocytes/macrophages. The middle cerebral artery was occluded for 60 min, followed by reperfusion. Hematopoietic prostaglandin D synthase expression was examined by immunohistochemistry and Western blotting. Hematopoietic prostaglandin D synthase-positive cells were mainly expressed in the peri-ischemic area at 12 h (P<0.05) and 24 h (P<0.001) after reperfusion, while they were mostly found in the transition area at 48-72 h postreperfusion (P<0.001). There was a significant increase in staining intensity as well as number of hematopoietic prostaglandin D synthase-positive cells in the ischemic core at 5-7 (P<0.001) days postreperfusion. Hematopoietic prostaglandin D synthase-positive cells also co-expressed ionized calcium-binding adapter molecule 1, a marker of microglia/macrophages, and cyclooxygenase-2, but not markers of neurons, oligodendrocytes and astrocytes. Until 72 h postreperfusion, many enhanced green fluorescent protein-positive cells were negative for hematopoietic prostaglandin D synthase, but the number of hematopoietic prostaglandin D synthase-enhanced green fluorescent protein coexpressing cells increased significantly at 5-7 days after reperfusion. Our results indicate that hematopoietic prostaglandin D synthase is mainly produced by endogenous microglia until 72 h after reperfusion, but at 7 days after reperfusion, it is also produced by migrating bone marrow/blood-derived macrophages in the ischemic brain tissue. We speculate that hematopoietic prostaglandin D synthase in the brain has different functions during early and late phases of ischemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
520-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17241746-Animals, pubmed-meshheading:17241746-Brain, pubmed-meshheading:17241746-Brain Ischemia, pubmed-meshheading:17241746-Calcium-Binding Proteins, pubmed-meshheading:17241746-Cell Count, pubmed-meshheading:17241746-Cell Movement, pubmed-meshheading:17241746-Cell Proliferation, pubmed-meshheading:17241746-Cyclooxygenase 2, pubmed-meshheading:17241746-Green Fluorescent Proteins, pubmed-meshheading:17241746-Hematopoiesis, pubmed-meshheading:17241746-Intramolecular Oxidoreductases, pubmed-meshheading:17241746-Lipocalins, pubmed-meshheading:17241746-Macrophages, pubmed-meshheading:17241746-Male, pubmed-meshheading:17241746-Mice, pubmed-meshheading:17241746-Mice, Inbred C57BL, pubmed-meshheading:17241746-Microfilament Proteins, pubmed-meshheading:17241746-Microglia, pubmed-meshheading:17241746-Prostaglandin D2, pubmed-meshheading:17241746-Reperfusion Injury, pubmed-meshheading:17241746-Transplantation Chimera, pubmed-meshheading:17241746-Up-Regulation
pubmed:year
2007
pubmed:articleTitle
Focal cerebral ischemia/reperfusion injury in mice induces hematopoietic prostaglandin D synthase in microglia and macrophages.
pubmed:affiliation
Department of Neurology, Juntendo University School of Medicine 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't