Linked Life Data

17241708

Source:http://linkedlifedata.com/resource/pubmed/id/17241708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2007-2-16
pubmed:abstractText
Due to the complex life cycle and high antigenic diversity of the malaria parasite, a multistage vaccine may be necessary for optimal protection against the disease. Our previous studies demonstrated that a blood-stage recombinant protein PfCP-2.9 has significant potential for vaccine development and is currently in human clinical trials. This study constructed two recombinant antigens derived from the Plasmodium falciparum CSP, designated PfCSP-C and PfCSP-RC. They were expressed as secreted proteins at high yield (1-3 g/l) in Pichia pastoris and purified by a two-step purification procedure. There was no evidence of antigen competition in mice and rabbits co-immunized with the pre-erythrocytic antigens and PfCP-2.9. Moreover, the immune sera recognized both the blood-stage parasite and sporozoite, and interacted with the NANP repeats of PfCSP. Rabbits antisera to combination antigens strongly inhibited blood-stage parasite growth in vitro. These results suggest that the recombinant antigens are potential candidates for multistage combination vaccines against malarial parasite.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2112-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Construction and evaluation of a multistage combination vaccine against malaria.
pubmed:affiliation
Department of Etiologic Biology and State Key Laboratory of Medical Immunology, Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't