Linked Life Data

17241111

Source:http://linkedlifedata.com/resource/pubmed/id/17241111

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-23
pubmed:abstractText
Immunosuppressive drugs can be completely withdrawn in up to 20% of liver transplant recipients, commonly referred to as 'operationally' tolerant. Immune characterization of these patients, however, has not been performed in detail, and we lack tests capable of identifying tolerant patients among recipients receiving maintenance immunosuppression. In the current study we have analyzed a variety of biological traits in peripheral blood of operationally tolerant liver recipients in an attempt to define a multiparameter 'fingerprint' of tolerance. Thus, we have performed peripheral blood gene expression profiling and extensive blood cell immunophenotyping on 16 operationally tolerant liver recipients, 16 recipients requiring on-going immunosuppressive therapy, and 10 healthy individuals. Microarray profiling identified a gene expression signature that could discriminate tolerant recipients from immunosuppression-dependent patients with high accuracy. This signature included genes encoding for gammadelta T-cell and NK receptors, and for proteins involved in cell proliferation arrest. In addition, tolerant recipients exhibited significantly greater numbers of circulating potentially regulatory T-cell subsets (CD4+ CD25+ T-cells and Vdelta1+ T cells) than either non-tolerant patients or healthy individuals. Our data provide novel mechanistic insight on liver allograft operational tolerance, and constitute a first step in the search for a non-invasive diagnostic signature capable of predicting tolerance before undergoing drug weaning.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1600-6135
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-19
pubmed:meshHeading
pubmed-meshheading:17241111-Antigens, CD4, pubmed-meshheading:17241111-DNA, pubmed-meshheading:17241111-DNA, Viral, pubmed-meshheading:17241111-Gene Expression Profiling, pubmed-meshheading:17241111-Graft Rejection, pubmed-meshheading:17241111-Hepacivirus, pubmed-meshheading:17241111-Humans, pubmed-meshheading:17241111-Immune Tolerance, pubmed-meshheading:17241111-Immunophenotyping, pubmed-meshheading:17241111-Immunosuppressive Agents, pubmed-meshheading:17241111-Interleukin-2 Receptor alpha Subunit, pubmed-meshheading:17241111-Liver Transplantation, pubmed-meshheading:17241111-Middle Aged, pubmed-meshheading:17241111-Predictive Value of Tests, pubmed-meshheading:17241111-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:17241111-T-Lymphocytes, Regulatory, pubmed-meshheading:17241111-Transplantation Immunology, pubmed-meshheading:17241111-Transplantation Tolerance
pubmed:year
2007
pubmed:articleTitle
Multiparameter immune profiling of operational tolerance in liver transplantation.
pubmed:affiliation
Liver Transplant Unit, Hospital Clinic Barcelona, IDIBAPS, University of Barcelona, Barcelona, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't