Linked Life Data

17240101

Source:http://linkedlifedata.com/resource/pubmed/id/17240101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-3-27
pubmed:abstractText
Under inflammatory conditions, both 8-nitroguanine (NO2Gua) and 8-hydroxydeoxyguanosine (8-OHdG) are found in tissues. Measurements of the two types of damaged bases on nucleotides are expected to provide information pointing to the possible correlation between inflammation and carcinogenesis. For the establishment of an in vivo model, in this study, a sensitive and precise method for the determination of NO2Gua, which uses liquid chromatography with mass spectrometry (LC-MS) and 6-methoxy-2-naphthyl glyoxal (MTNG) derivatization, was developed in vitro. The procedure for DNA digestion in this method is identical to that widely used for 8-OHdG measurement, which enables us to detect the two damaged bases in the same DNA sample. In order to validate our method, we measured NO2Gua levels in DNA sample using LC-MS. A mass spectrometer equipped with an electrospray atmospheric pressure ionization source and operated in the negative ion mode (ESI-) was set up with selective ion monitoring at m/z 391 and 394 for NO2Gua-MTNG and [13C, 15N2]-NO2Gua-MTNG as surrogate standard, respectively. The average recoveries from DNA samples spiked with 25, 50 and 250 nM NO2Gua were 99.4, 99.8 and 99.1% with correction using the added surrogate standard, respectively. The limit of quantification was 3.0 nM for NO2Gua. To ascertain the applicability of our method to DNA samples harboring the two damaged bases, we measured NO2Gua and 8-OHdG levels in calf thymus DNA treated with ONOO-. As a result, both NO2Gua and 8-OHdG levels were clearly increased with ONOO- dose dependency, the amount of NO2Gua at the high dose ONOO- being almost the same as those of 8-OHdG. LC-MS was able to determine NO2Gua in a small amount of DNA sample, and is therefore expected to be a very powerful tool for the evaluation of DNA damage induced by reactive nitrogen species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1737-43
pubmed:meshHeading
pubmed-meshheading:17240101-Animals, pubmed-meshheading:17240101-Buffers, pubmed-meshheading:17240101-Cattle, pubmed-meshheading:17240101-Chelating Agents, pubmed-meshheading:17240101-Chromatography, Liquid, pubmed-meshheading:17240101-DNA, pubmed-meshheading:17240101-DNA Damage, pubmed-meshheading:17240101-Deoxyguanosine, pubmed-meshheading:17240101-Dose-Response Relationship, Drug, pubmed-meshheading:17240101-Glyoxal, pubmed-meshheading:17240101-Guanine, pubmed-meshheading:17240101-Hydrogen-Ion Concentration, pubmed-meshheading:17240101-Mass Spectrometry, pubmed-meshheading:17240101-Molecular Structure, pubmed-meshheading:17240101-Oxidants, pubmed-meshheading:17240101-Pentetic Acid, pubmed-meshheading:17240101-Peroxynitrous Acid, pubmed-meshheading:17240101-Reproducibility of Results, pubmed-meshheading:17240101-Sensitivity and Specificity, pubmed-meshheading:17240101-Spectrometry, Mass, Electrospray Ionization, pubmed-meshheading:17240101-Spectrophotometry, Ultraviolet, pubmed-meshheading:17240101-Temperature, pubmed-meshheading:17240101-Thymus Gland, pubmed-meshheading:17240101-Time Factors
pubmed:year
2007
pubmed:articleTitle
Development of quantitative analysis of 8-nitroguanine concomitant with 8-hydroxydeoxyguanosine formation by liquid chromatography with mass spectrometry and glyoxal derivatization.
pubmed:affiliation
Department of Analytical Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't