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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1992-3-26
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pubmed:abstractText |
Confocal imaging was performed on the ceratomandibularis nerve muscle preparation of the lizard Anolis carolinensis, using 4-Di-2-ASP as a fluorescent probe. The imaging system consisted of a Sarastro Phoibos 1000 (Molecular Dynamics) scanning system and a Zeiss Universal microscope. The data were analyzed using the VANIS set of programs on a Silicon Graphics Personal Iris computer. A three dimensional reconstruction of the nerve terminals was performed using look-through and depth-coded projections. The volume of the nerve terminal was estimated in 15 neuromuscular junctions and found to be 84,835 microns 3 (+/- 8558 SEM). The largest diameter in each one of the 178 individual boutons was estimated from the look-through projections of 17 nerve terminals in 9 preparations. It was found to be 4.71 microns (+/- 0.08 SEM). The diameter perpendicular to the largest diameter in the same projection at 0 degrees was 3.3 microns (+/- 0.054 SEM). Thus it seems that the synaptic boutons of the ceratomandibularis are suitable for combined optical and electrophysiological recordings.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0792-6855
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-85
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1723895-Animals,
pubmed-meshheading:1723895-Fluorescent Dyes,
pubmed-meshheading:1723895-Lizards,
pubmed-meshheading:1723895-Mitochondria,
pubmed-meshheading:1723895-Motor Neurons,
pubmed-meshheading:1723895-Nerve Endings,
pubmed-meshheading:1723895-Neuromuscular Junction,
pubmed-meshheading:1723895-Pyridinium Compounds,
pubmed-meshheading:1723895-Staining and Labeling,
pubmed-meshheading:1723895-Synapses
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pubmed:articleTitle |
Confocal microscopy of the lizard motor nerve terminals.
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pubmed:affiliation |
Department of Physiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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