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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-2-16
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pubmed:abstractText |
Cytotoxic T lymphocytes and natural killer cells exert their cytotoxic activity through the polarized secretion of cytotoxic granules at the immunological synapse. Rab27a and hMunc13-4 are critical effectors of the exocytosis of cytotoxic granules. Here we show that the cytotoxic function of lymphocytes requires the cooperation of two types of organelles: the lysosomal cytotoxic granule and the endosomal 'exocytic vesicle'. Independently of Rab27a, hMunc13-4 mediated the assembly of Rab11(+) recycling and Rab27(+) late endosomal vesicles, constituting a pool of vesicles destined for regulated exocytosis. It also primed cytotoxic granule fusion, possibly through interaction with active Rab27a. Cytotoxic T lymphocyte-target cell recognition induced rapid polarization of both types of organelles, which coalesced near the cell-cell contact area. Our data provide insight into the regulation of the generation and release of cytotoxic granules by effector cytotoxic T lymphocytes and natural killer cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1529-2908
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
257-67
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pubmed:dateRevised |
2007-11-8
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pubmed:meshHeading |
pubmed-meshheading:17237785-Cell Polarity,
pubmed-meshheading:17237785-Cells, Cultured,
pubmed-meshheading:17237785-Cytotoxicity, Immunologic,
pubmed-meshheading:17237785-Endosomes,
pubmed-meshheading:17237785-Exocytosis,
pubmed-meshheading:17237785-Fluorescent Antibody Technique,
pubmed-meshheading:17237785-Humans,
pubmed-meshheading:17237785-Immunoblotting,
pubmed-meshheading:17237785-Immunoprecipitation,
pubmed-meshheading:17237785-Killer Cells, Natural,
pubmed-meshheading:17237785-Lymphocyte Activation,
pubmed-meshheading:17237785-Lysosomes,
pubmed-meshheading:17237785-Microscopy, Electron, Transmission,
pubmed-meshheading:17237785-Microscopy, Immunoelectron,
pubmed-meshheading:17237785-Nerve Tissue Proteins,
pubmed-meshheading:17237785-Polymerase Chain Reaction,
pubmed-meshheading:17237785-Secretory Vesicles,
pubmed-meshheading:17237785-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:17237785-Transfection,
pubmed-meshheading:17237785-rab GTP-Binding Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Secretory cytotoxic granule maturation and exocytosis require the effector protein hMunc13-4.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale Unité 768, Laboratoire du Développement Normal et Pathologique du Système Immunitaire, Paris F-75015 France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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