Source:http://linkedlifedata.com/resource/pubmed/id/17235319
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2007-1-19
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pubmed:abstractText |
Recent studies have indicated that type I interferon (IFN) enhances antibody responses and promotes isotype switching. In this study, we analyzed the role of type I IFN signaling during the generation of transgene product-specific antibody responses elicited by recombinant adenovirus (Ad) vectors. A vector derived from a human Ad serotype (AdHu5) induced low levels of type I IFN following infection of dendritic cells (DCs) and stimulated normal transgene product-specific antibody responses in mice that have a defective type I IFN receptor (IFNAR(-/-)). A vector derived from a chimpanzee Ad serotype (AdC68) induced very high levels of type I IFN following infection of DCs, and surprisingly, primed stronger transgene product-specific antibody responses in IFNAR(-/-) mice compared to wild-type mice. The increased antibody response in IFNAR(-/-) mice vaccinated with the AdC68 vector was mainly due to the generation of IgG1 antibodies that were not elicited in wild-type mice. The induction of IgG1 antibodies correlated with an increase in transgene product expression in IFNAR(-/-) mice and was not associated with an increase in T helper 2 responses. We conclude that type I IFN, when induced at high levels, can downregulate transgene product expression of Ad vectors and inhibit the formation of optimal antibody responses.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1525-0016
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
393-403
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pubmed:dateRevised |
2007-12-3
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pubmed:meshHeading |
pubmed-meshheading:17235319-Adenoviridae,
pubmed-meshheading:17235319-Animals,
pubmed-meshheading:17235319-Bone Marrow Cells,
pubmed-meshheading:17235319-Cytokines,
pubmed-meshheading:17235319-Dendritic Cells,
pubmed-meshheading:17235319-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17235319-Genetic Vectors,
pubmed-meshheading:17235319-Humans,
pubmed-meshheading:17235319-Immunoglobulin G,
pubmed-meshheading:17235319-Interferon Type I,
pubmed-meshheading:17235319-Mice,
pubmed-meshheading:17235319-Mice, Inbred C57BL,
pubmed-meshheading:17235319-Mice, Knockout,
pubmed-meshheading:17235319-Pan troglodytes,
pubmed-meshheading:17235319-Receptor, Interferon alpha-beta,
pubmed-meshheading:17235319-Signal Transduction,
pubmed-meshheading:17235319-Th2 Cells
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pubmed:year |
2007
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pubmed:articleTitle |
Type I interferon inhibits antibody responses induced by a chimpanzee adenovirus vector.
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pubmed:affiliation |
Cell and Molecular Biology Group, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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