Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-1-31
pubmed:abstractText
The approximately 80-kb pksX gene cluster in Bacillus subtilis encodes an unusual hybrid polyketide/nonribosomal peptide synthase that has been linked to the production of the uncharacterized antibiotic bacillaene. Multiple copies of this synthase, each similar in size to the ribosome, assemble into a single organelle-like complex with a mass of tens to hundreds of megadaltons. The resource requirements of the assembled megacomplex suggest that bacillaene has an important biological role. By coupling a differential NMR spectroscopic technique with genetically manipulated strains of B. subtilis, we were able to characterize the structure of this unusual secondary metabolite, which could not be predicted by using bioinformatic analysis. We report that bacillaene is a linear molecule with two amide bonds: the first links an alpha-hydroxy carboxylic acid to a omega-amino carboxylic acid containing a conjugated hexaene, and the second links the hexaene-containing carboxylic acid to an (omega-1) amino carboxylic acid containing a conjugated triene. Knowledge of bacillaene's structure has enabled us to annotate the pksX gene cluster and should facilitate the study of bacillaene's biosynthesis as well as its biological role in B. subtilis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-11578925, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-12515540, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-12851937, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-15217615, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-15332855, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-15844935, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-15880675, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16084035, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16107321, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16408055, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16460000, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16707694, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16757561, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16834357, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16895337, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-16910662, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-17190806, http://linkedlifedata.com/resource/pubmed/commentcorrection/17234808-7592068
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1506-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis.
pubmed:affiliation
Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural