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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-3-13
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pubmed:abstractText |
There is a need for simple, quantitative and prospective assays for islet quality assessment that are predictive of islet transplantation outcome. The current state-of-the-art athymic nude mouse bioassay is costly, technically challenging and retrospective. In this study, we report on the ability of 2 parameters characterizing human islet quality: (1) oxygen consumption rate (OCR), a measure of viable volume; and (2) OCR/DNA, a measure of fractional viability, to predict diabetes reversal in nude mice. Results demonstrate that the probability for diabetes reversal increases as the graft's OCR/DNA and total OCR increase. For a given transplanted OCR dose, diabetes reversal is strongly dependent on OCR/DNA. The OCR and OCR/DNA (the 'OCR test') data exhibit 89% sensitivity and 77% specificity in predicting diabetes reversal in nude mice (n = 86). We conclude that the prospective OCR test can effectively replace the retrospective athymic nude mouse bioassay in assessing human islet quality prior to islet transplantation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-10083280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-10556766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-10578092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-10752741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-10911004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-11120194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-11267479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-11797699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-12450449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-12796569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-12947315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-15713772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-15943621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-15996257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-16033685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-16280654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-16298611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-16298642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-2088991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-2699586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17229069-9000675
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1600-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
707-13
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:17229069-Animals,
pubmed-meshheading:17229069-Biological Assay,
pubmed-meshheading:17229069-Blood Glucose,
pubmed-meshheading:17229069-Cell Survival,
pubmed-meshheading:17229069-DNA,
pubmed-meshheading:17229069-Diabetes Mellitus,
pubmed-meshheading:17229069-Humans,
pubmed-meshheading:17229069-Islets of Langerhans,
pubmed-meshheading:17229069-Islets of Langerhans Transplantation,
pubmed-meshheading:17229069-Mice,
pubmed-meshheading:17229069-Mice, Nude,
pubmed-meshheading:17229069-Oxygen Consumption,
pubmed-meshheading:17229069-Prognosis,
pubmed-meshheading:17229069-Treatment Outcome
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pubmed:year |
2007
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pubmed:articleTitle |
Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice.
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pubmed:affiliation |
Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota, Minneapolis, MN, USA. papas006@umn.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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