Source:http://linkedlifedata.com/resource/pubmed/id/17227766
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2007-3-12
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| pubmed:abstractText |
The junctional adhesion molecule (JAM) family is a key molecule in a process called transendothelial migration or diapedesis. Here, we report implications of JAM-C in cancer metastasis. We first determined the mRNA expression of JAMs in 19 kinds of cancer cell lines. JAM-C was expressed in most of tumors having potent metastatic properties. Especially in murine K-1735 melanoma cell lines, the highly metastatic sublines (M2 and X21) strongly expressed JAM-C when compared with the poorly metastatic ones (C-10 and C23). Next, we investigated the role of JAM-C in cancer metastasis by using human JAM-C (hJAM-C) gene-transfected HT1080 fibrosarcoma cells. In comparison with mock-transfected HT1080 cells, these cells showed a significant increase in the adhesion to various extracellular substrates and the invasion across a Matrigel-coated membrane. The knockdown of hJAM-C using small interfering RNA resulted in the suppression of both the adhesion and the invasion of HT1080 cells, suggesting that endogenous hJAM-C might be involved in tumor metastasis. Finally, we studied the role of hJAM-C in an in vivo experimental metastatic model. The results showed that the overexpression of hJAM-C in HT1080 cells significantly decreased the life spans of the tumorbearing mice. In contrast, the knockdown of hJAM-C in HT1080 cells suppressed the weight gain of the lungs with metastatic colonies. We conclude that the expression of JAM-C promotes metastasis by enhancing both the adhesion of cancer cells to extracellular matrices and the subsequent invasion.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/JAM3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Jam3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/matrigel
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
16
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| pubmed:volume |
282
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
8276-83
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| pubmed:meshHeading |
pubmed-meshheading:17227766-Animals,
pubmed-meshheading:17227766-CHO Cells,
pubmed-meshheading:17227766-Cell Adhesion,
pubmed-meshheading:17227766-Cell Adhesion Molecules,
pubmed-meshheading:17227766-Cell Line, Tumor,
pubmed-meshheading:17227766-Collagen,
pubmed-meshheading:17227766-Cricetinae,
pubmed-meshheading:17227766-Cricetulus,
pubmed-meshheading:17227766-Drug Combinations,
pubmed-meshheading:17227766-Humans,
pubmed-meshheading:17227766-Immunoglobulins,
pubmed-meshheading:17227766-Laminin,
pubmed-meshheading:17227766-Lung Neoplasms,
pubmed-meshheading:17227766-Male,
pubmed-meshheading:17227766-Membrane Proteins,
pubmed-meshheading:17227766-Mice,
pubmed-meshheading:17227766-Neoplasm Invasiveness,
pubmed-meshheading:17227766-Neoplasm Metastasis,
pubmed-meshheading:17227766-Prostatic Neoplasms,
pubmed-meshheading:17227766-Proteoglycans
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| pubmed:year |
2007
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| pubmed:articleTitle |
Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
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| pubmed:affiliation |
Department of Medical Biochemistry and Center of Excellence Program in the 21st Century, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
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| pubmed:publicationType |
Journal Article
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