Linked Life Data

17226753

Source:http://linkedlifedata.com/resource/pubmed/id/17226753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-4-2
pubmed:abstractText
To investigate the molecular mechanism underlying the differentiation of osteoblasts and chondroblasts, we established a clonal cell lines, RD-C6, from Runx2-deficient mouse embryos. RD-C6 cells expressed almost undetectable levels of phenotypes related to osteoblast and chondroblast differentiation at basal culture condition, whereas treatment with recombinant human bone morphogenetic protein-2 (rhBMP-2) or transduction of BMP-2 by adenovirus effectively induced this cell line to express mRNA related to the differentiation of osteoblasts and chondroblasts including alkaline phosphatase, osteocalcin, and osterix. Transduction of Runx2 also induced the expression of these mRNA in RD-C6 cells. BMP-2 transduction increased expression levels of mRNA for Msx2 and Dlx5, but Runx2 transduction induced no significant increases in expression levels of these mRNA. Microarray analysis using RD-C6 cells with or without rhBMP-2 treatment demonstrated that BMP-2 upregulated 66 genes including 13 transcription-related molecules such as Id1, Id2, Id4, Hey1, Smad6, Smad7, and Msx2. To confirm bone and cartilage formation ability of RD-C6 cells, we transplanted RD-C6 cells into the peritoneal cavity of athymic mice using diffusion chambers with rhBMP-2. RD-C6 cells generated unmineralized cartilage but not bone. These results indicate that BMP-2 induces Runx2-deficient cells to express markers related to osteoblast and chondroblast differentiation using a Runx2-independent pathway, but it failed to induce these cells to differentiate into bone-forming osteoblasts and mature chondrocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
211
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
728-35
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17226753-Animals, pubmed-meshheading:17226753-Bone Morphogenetic Protein 2, pubmed-meshheading:17226753-Bone Morphogenetic Proteins, pubmed-meshheading:17226753-Cartilage, pubmed-meshheading:17226753-Cell Differentiation, pubmed-meshheading:17226753-Cell Line, pubmed-meshheading:17226753-Chondrocytes, pubmed-meshheading:17226753-Core Binding Factor Alpha 1 Subunit, pubmed-meshheading:17226753-Diffusion Chambers, Culture, pubmed-meshheading:17226753-Gene Expression, pubmed-meshheading:17226753-Mice, pubmed-meshheading:17226753-Mice, Mutant Strains, pubmed-meshheading:17226753-Mice, Nude, pubmed-meshheading:17226753-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17226753-Osteoblasts, pubmed-meshheading:17226753-Peritoneal Cavity, pubmed-meshheading:17226753-Phenotype, pubmed-meshheading:17226753-Skull, pubmed-meshheading:17226753-Transforming Growth Factor beta, pubmed-meshheading:17226753-Transplants
pubmed:year
2007
pubmed:articleTitle
BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.
pubmed:affiliation
Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't