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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1992-2-20
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pubmed:abstractText |
The nephritogenic antigen that induces antiglomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in rats was isolated from collagenase-solubilized bovine renal basement membranes. Purification was achieved using antibody-coupled affinity columns which were originally used for the purification of trypsin-solubilized nephritogenic antigen (Sado et al. 1984a). The nephritogenic antigen was a heteropolymer composed of P2 (Mr 28 kDa) and P3 (Mr 30 kDa) polypeptides as monomers and their dimers in sodium-dodecyl-sulfate (SDS) polyacrylamide gel electrophoresis. The P3 polypeptide was considered to be the nephritogenic epitope, since a fraction composed of the P2 polypeptide alone was not nephritogenic. The properties of the nephritogenic epitope were the same as those of the Goodpasture epitope (M2*), which is a noncollagenous domain of the alpha 3 chain of type IV collagen (Butkowski et al. 1985; Saus et al. 1988), indicating that the nephritogenic antigen is the same as the Goodpasture antigen.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0340-6075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
345-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1722595-Animals,
pubmed-meshheading:1722595-Anti-Glomerular Basement Membrane Disease,
pubmed-meshheading:1722595-Antibodies,
pubmed-meshheading:1722595-Autoantigens,
pubmed-meshheading:1722595-Autoimmune Diseases,
pubmed-meshheading:1722595-Basement Membrane,
pubmed-meshheading:1722595-Cattle,
pubmed-meshheading:1722595-Chromatography, Affinity,
pubmed-meshheading:1722595-Chromatography, Gel,
pubmed-meshheading:1722595-Collagen,
pubmed-meshheading:1722595-Collagen Type IV,
pubmed-meshheading:1722595-Disease Models, Animal,
pubmed-meshheading:1722595-Epitopes,
pubmed-meshheading:1722595-Female,
pubmed-meshheading:1722595-Glomerulonephritis,
pubmed-meshheading:1722595-Heymann Nephritis Antigenic Complex,
pubmed-meshheading:1722595-Humans,
pubmed-meshheading:1722595-Immunodiffusion,
pubmed-meshheading:1722595-Kidney Glomerulus,
pubmed-meshheading:1722595-Membrane Glycoproteins,
pubmed-meshheading:1722595-Microbial Collagenase,
pubmed-meshheading:1722595-Rats,
pubmed-meshheading:1722595-Rats, Inbred WKY,
pubmed-meshheading:1722595-Trypsin
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pubmed:year |
1991
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pubmed:articleTitle |
Properties of bovine nephritogenic antigen that induces anti-GBM nephritis in rats and its similarity to the Goodpasture antigen.
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pubmed:affiliation |
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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