| pubmed-article:1722493 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0005220 | lld:lifeskim |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0019941 | lld:lifeskim |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0242349 | lld:lifeskim |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0301869 | lld:lifeskim |
| pubmed-article:1722493 | lifeskim:mentions | umls-concept:C0597551 | lld:lifeskim |
| pubmed-article:1722493 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:1722493 | pubmed:dateCreated | 1992-2-14 | lld:pubmed |
| pubmed-article:1722493 | pubmed:abstractText | Synthetic peptide-alkaline phosphatase conjugates can be used to detect the epitope specificity of (i) antibody-forming cells in vivo by immunocytochemistry; (ii) of antibody secreting cells in vitro by spot-ELISA; and (iii) antibodies in solution by capture ELISA. The availability of synthetic peptide-enzyme conjugates using detector enzymes other than alkaline phosphatase would offer several important advantages, for example in double staining approaches. This paper reports the production of synthetic peptide-horseradish peroxidase conjugates and synthetic peptide-beta-galactosidase conjugates. A peptide of 21 amino acids (SP 29) was coupled to peroxidase in seven differing molar ratios of peptide over peroxidase, ranging from 1:3.4 to 1:575, using periodate oxidation of the enzyme. SP 29 was coupled to beta-galactosidase in four molar ratios ranging from 1.25 to 10, using glutaraldehyde pre-activation of the enzyme. The enzyme activity of the different conjugates was determined, the conjugates were tested in direct capture-ELISA with peptide-specific monoclonal antibodies, and the conjugates were tested in immunocytochemistry to detect peptide-specific B cells. The results show that the conjugates perform best if the peptide is coupled to the enzyme at relatively low molar ratios (1-30). The availability of these new peptide-enzyme conjugates broadens the applicability of synthetic peptides for detection purposes in several assay systems. | lld:pubmed |
| pubmed-article:1722493 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1722493 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1722493 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1722493 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1722493 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:ClaassenEE | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:LamanJ DJD | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:BoersmaW JWJ | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:van den... | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:DeegJJ | lld:pubmed |
| pubmed-article:1722493 | pubmed:author | pubmed-author:VermeulenNN | lld:pubmed |
| pubmed-article:1722493 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1722493 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1722493 | pubmed:volume | 145 | lld:pubmed |
| pubmed-article:1722493 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1722493 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1722493 | pubmed:pagination | 1-10 | lld:pubmed |
| pubmed-article:1722493 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:meshHeading | pubmed-meshheading:1722493-... | lld:pubmed |
| pubmed-article:1722493 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1722493 | pubmed:articleTitle | Synthetic peptide conjugates with horseradish peroxidase and beta-galactosidase for use in epitope-specific immunocytochemistry and ELISA. | lld:pubmed |
| pubmed-article:1722493 | pubmed:affiliation | Department of Immunology and Medical Microbiology, TNO Medical Biological Laboratory, Rijswijk, The Netherlands. | lld:pubmed |
| pubmed-article:1722493 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1722493 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1722493 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1722493 | lld:pubmed |
