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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-25
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pubmed:abstractText |
In this study we have combined fluorescence- and reflection-confocal laser scanning microscopy for the simultaneous visualization of living cells and surface topography beneath them. To this purpose we have designed a specific flow chamber and we have tested it with osteoblasts grown on an opaque, thick support, made of smooth or sandblasted titanium. Cells were loaded with Calcein-AM or tetramethylrhodamine methyl ester (TMRM), two probes employed as indicators of cell viability/morphology and mitochondrial membrane potential, respectively. Besides the acquisition of stacks of confocal sections, the system allowed also vertical views and faithful three-dimensional reconstruction of the samples. Confocal microscope implemented with our flow chamber proved to be a promising tool for time-lapse investigation of cell-biomaterial interactions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0968-4328
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
137-43
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pubmed:meshHeading |
pubmed-meshheading:17223563-Biocompatible Materials,
pubmed-meshheading:17223563-Cells, Cultured,
pubmed-meshheading:17223563-Fluoresceins,
pubmed-meshheading:17223563-Fluorescent Dyes,
pubmed-meshheading:17223563-Humans,
pubmed-meshheading:17223563-Imaging, Three-Dimensional,
pubmed-meshheading:17223563-Microscopy, Confocal,
pubmed-meshheading:17223563-Microscopy, Fluorescence,
pubmed-meshheading:17223563-Osteoblasts,
pubmed-meshheading:17223563-Rhodamines,
pubmed-meshheading:17223563-Surface Properties,
pubmed-meshheading:17223563-Titanium
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pubmed:year |
2008
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pubmed:articleTitle |
Analysis of living cells grown on different titanium surfaces by time-lapse confocal microscopy.
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pubmed:affiliation |
Department of Experimental Medicine, University of Parma, Via Volturno 39, 43100 Parma, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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