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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-3-22
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pubmed:abstractText |
In this study we examined mechanisms that regulate T-helper lymphocyte 1 (Th1) commitment in Helicobacter pylori-infected human gastric mucosa. The levels of gamma interferon (IFN-gamma), interleukin-4 (IL-4), and IL-12 in total extracts of gastric biopsies taken from H. pylori-infected and uninfected patients were determined by an enzyme-linked immunosorbent assay. The levels of signal transducer and activator of transcription 4 (STAT4), STAT6, and T-box expressed in T cells (T-bet) in total proteins extracted from gastric biopsies were determined by Western blotting. Finally, the effect of a neutralizing IL-12 antibody on expression of Th1 transcription factors and the levels of IFN-gamma in organ cultures of H. pylori-infected biopsies was examined. Increased levels of IFN-gamma and IL-12 were found in gastric biopsy samples of H. pylori-infected patients compared to the levels in uninfected patients. In addition, H. pylori-infected biopsies exhibited high levels of expression of phosphorylated STAT4 and T-bet. Higher levels of IFN-gamma and expression of Th1 transcription factors were associated with greater infiltration of mononuclear cells in the gastric mucosa. By contrast, production of IL-4 and expression of phosphorylated STAT6 were not associated with the intensity of mononuclear cell infiltration. In ex vivo organ cultures of H. pylori-infected biopsies, neutralization of endogenous IL-12 down-regulated the expression of phosphorylated STAT4 and T-bet and reduced IFN-gamma production. Our data indicated that IL-12 contributes to the Th1 cell commitment in H. pylori-infected human gastric mucosa.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-10358170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-10444264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-11114383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-11397944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-11786644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-12370372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-12461566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-12500979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-15153495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-16543949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-7638186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-7698689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-7905508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-7907633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-7994020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-8700208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-8827022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-8943050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-8943379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-8993017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9053967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9120387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9120388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9160750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9174613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9181473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220306-9743537
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/STAT4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/T-Box Domain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/T-box transcription factor TBX21
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1738-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17220306-Adult,
pubmed-meshheading:17220306-Aged,
pubmed-meshheading:17220306-Antibodies,
pubmed-meshheading:17220306-Biopsy,
pubmed-meshheading:17220306-Blotting, Western,
pubmed-meshheading:17220306-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17220306-Female,
pubmed-meshheading:17220306-Gastric Mucosa,
pubmed-meshheading:17220306-Helicobacter Infections,
pubmed-meshheading:17220306-Helicobacter pylori,
pubmed-meshheading:17220306-Humans,
pubmed-meshheading:17220306-Interferon-gamma,
pubmed-meshheading:17220306-Interleukin-12,
pubmed-meshheading:17220306-Interleukin-4,
pubmed-meshheading:17220306-Male,
pubmed-meshheading:17220306-Middle Aged,
pubmed-meshheading:17220306-Organ Culture Techniques,
pubmed-meshheading:17220306-STAT4 Transcription Factor,
pubmed-meshheading:17220306-STAT6 Transcription Factor,
pubmed-meshheading:17220306-Signal Transduction,
pubmed-meshheading:17220306-T-Box Domain Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Interleukin-12 drives the Th1 signaling pathway in Helicobacter pylori-infected human gastric mucosa.
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pubmed:affiliation |
Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro Magna Graecia, Campus Universitario di Germaneto, Viale Europa, 88100 Catanzaro, Italy.
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pubmed:publicationType |
Journal Article
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