Linked Life Data

17217952

Source:http://linkedlifedata.com/resource/pubmed/id/17217952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-2-12
pubmed:abstractText
Ion-pairing reversed-phase high-performance liquid chromatography (RP-HPLC) operation conditions were studied to obtain information useful for development and optimization of separation methods suitable for HPLC/MS analysis of dyes. The retention of eight sulphonated azodyes with widely differing structures (1-5 acid groups, molecular weight range 350-1220) was measured in mobile phases containing various ion-pairing reagents in aqueous-methanolic mobile phases. The effects of the type and of the concentration of ammonium acetate, tetrabutylammonium hydrogen sulphate and five di- and tri-alkylammonium acetate ion-pairing reagents on the chromatographic behaviour of dyes were compared in mobile phases with varying concentrations of methanol. Structural effects on the retention of dyes were studied in detail. The retention scale based on lipophilic and polar indices can be used for optimization of mobile phase for HPLC/MS of dyes and, on the other hand, may provide some information on the structure of unknown dyes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
1143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
112-20
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Behaviour of sulphonated azodyes in ion-pairing reversed-phase high-performance liquid chromatography.
pubmed:affiliation
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám. Cs. Legií 565, CZ-532 10 Pardubice, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't