Linked Life Data

1721027

Source:http://linkedlifedata.com/resource/pubmed/id/1721027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-1-23
pubmed:abstractText
The sialyl Lewis-x determinant is a ligand for ELAM-1, a major adhesion molecule for HL60 cells and neutrophils. ELAM-1 expression can selectively be induced on human umbilical vein endothelial cells (HUVEC) by tumor necrosis factor, interleukin 1 and lipopolysaccharide. The determinant sialyl Lewis-x is found on both glycolipids as well as on glycoproteins. Using specific inhibitors of the biosynthesis of N-linked glycosylated glycoproteins, we investigated whether N-linked glycans or their modifications are involved in ELAM-1-dependent adhesion of HL60 cells to activated HUVEC. The inhibitors of glycoprotein processing N-methyl-deoxynojirimycin, 1-deoxymannojirimycin and swainsonine did not affect ELAM-1-dependent adhesion. Complex-type N-linked glycans are not required for ELAM-1 mediated adhesion, and therefore the ligand for ELAM-1 is most likely a glycolipid, or a glycoprotein carrying O-linked oligosaccharides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3057-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
The ligand recognized by ELAM-1 on HL60 cells is not carried by N-linked oligosaccharides.
pubmed:affiliation
Department of Surgery, University of Limburg, Maastricht, The Netherlands.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't