Linked Life Data

17209058

Source:http://linkedlifedata.com/resource/pubmed/id/17209058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-4-23
pubmed:abstractText
The phenotype and function of murine dendritic cells (DCs) are primarily studied using bone-marrow-derived DCs (BM-DCs), but may be hampered by the heterogeneous phenotype of BM-DCs due to their differential state of maturation. Here we characterize a newly established murine DC line (SP37A3) of myeloid origin. During maintainance in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and M-CSF, SP37A3 cells resemble immature DCs characterized by low expression of major histocompatibility complex (MHC) II and costimulatory molecules and low T-cell stimulatory capacity. Upon stimulation, SP37A3 cells acquire a mature phenotype and activate naive T cells as potently as BM-DCs. Similar to BM-DCs, SP37A3 cells activated in the presence of dexamethasone-induced regulatory T cells, which were anergic upon restimulation and suppressed proliferation of naive T cells. This tolerogenic state was reflected by lower expression levels of costimulatory molecules and proinflammatory cytokines compared with mature cells, as well as up-regulated expression of FcgammaRIIB and interleukin-1RA (IL-1RA). SP37A3 cells were responsive to dexamethasone even when applied at later time points during activation, suggesting functional plasticity. Thus, DC line SP37A3 represents a suitable model to study functions of immature and mature as well as tolerogenic myeloid DCs, circumventing restrictions associated with the use of primary DCs and BM-DCs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3820-9
pubmed:meshHeading
pubmed-meshheading:17209058-Animals, pubmed-meshheading:17209058-Cell Differentiation, pubmed-meshheading:17209058-Cell Line, pubmed-meshheading:17209058-Cell Proliferation, pubmed-meshheading:17209058-Clonal Anergy, pubmed-meshheading:17209058-Coculture Techniques, pubmed-meshheading:17209058-Cytokines, pubmed-meshheading:17209058-Dendritic Cells, pubmed-meshheading:17209058-Dexamethasone, pubmed-meshheading:17209058-Glucocorticoids, pubmed-meshheading:17209058-Histocompatibility Antigens Class II, pubmed-meshheading:17209058-Interleukin 1 Receptor Antagonist Protein, pubmed-meshheading:17209058-Mice, pubmed-meshheading:17209058-Mice, Inbred BALB C, pubmed-meshheading:17209058-Myeloid Progenitor Cells, pubmed-meshheading:17209058-Receptors, IgG, pubmed-meshheading:17209058-T-Lymphocytes, Regulatory, pubmed-meshheading:17209058-Up-Regulation
pubmed:year
2007
pubmed:articleTitle
A newly established murine immature dendritic cell line can be differentiated into a mature state, but exerts tolerogenic function upon maturation in the presence of glucocorticoid.
pubmed:affiliation
Clinical Research Unit Allergology, Department of Dermatology, Johannes Gutenberg-University, Obere Zahlbacher Strasse 63, D-55131 Mainz, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't