|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2007-3-22
|
|
pubmed:abstractText |
We have investigated the ability of apoE (apolipoprotein E) to participate in the biogenesis of HDL (high-density lipoprotein) particles in vivo using adenovirus-mediated gene transfer in apoA-I-/- (apolipoprotein A-I) or ABCA1-/- (ATP-binding cassette A1) mice. Infection of apoA-I-/- mice with 2x10(9) pfu (plaque-forming units) of an apoE4-expressing adenovirus increased both HDL and the triacylglycerol-rich VLDL (very-low-density lipoprotein)/IDL (intermediate-density lipoprotein)/LDL (low-density lipoprotein) fraction and generated discoidal HDL particles. ABCA1-/- mice treated similarly failed to form HDL particles, suggesting that ABCA1 is essential for the generation of apoE-containing HDL. Combined infection of apoA-I-/- mice with a mixture of adenoviruses expressing both apoE4 (2x10(9) pfu) and human LCAT (lecithin:cholesterol acyltransferase) (5x10(8) pfu) cleared the triacylglycerol-rich lipoproteins, increased HDL and converted the discoidal HDL into spherical HDL. Similarly, co-infection of apoE-/- mice with apoE4 and human LCAT corrected the hypercholesterolaemia and generated spherical particles, suggesting that LCAT is essential for the maturation of apoE-containing HDL. Overall, the findings indicate that apoE has a dual functionality. In addition to its documented functions in the clearance of triacylglycerol-rich lipoproteins, it participates in the biogenesis of HDL-sized apoE-containing particles. HDL particles generated by this pathway may account at least for some of the atheroprotective functions of apoE.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10327277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10330390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10430941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10479659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10760292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10933930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10938015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-10974056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-11279066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-11433352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-11861414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-11882653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-12488454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-12924933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-1411543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-1423598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-14754908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-1477274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-1496008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15017358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15269217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15576362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15591220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15654758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-15751988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-16185081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-16207707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-16207713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-16245952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-16339113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-182724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-2597674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-3771793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-4054131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-7400692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-7440719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-7860728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-8127890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-8241102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17206937-9327769
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP binding cassette transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein A-I,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholine-Sterol...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/apolipoprotein E-rich HDL
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1470-8728
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
15
|
|
pubmed:volume |
403
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
359-67
|
|
pubmed:dateRevised |
2011-2-7
|
|
pubmed:meshHeading |
pubmed-meshheading:17206937-ATP-Binding Cassette Transporters,
pubmed-meshheading:17206937-Adenoviridae,
pubmed-meshheading:17206937-Alanine,
pubmed-meshheading:17206937-Animals,
pubmed-meshheading:17206937-Apolipoprotein A-I,
pubmed-meshheading:17206937-Apolipoproteins E,
pubmed-meshheading:17206937-Humans,
pubmed-meshheading:17206937-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:17206937-Lipoproteins, HDL,
pubmed-meshheading:17206937-Metabolic Networks and Pathways,
pubmed-meshheading:17206937-Mice,
pubmed-meshheading:17206937-Mice, Knockout,
pubmed-meshheading:17206937-Phosphatidylcholine-Sterol O-Acyltransferase,
pubmed-meshheading:17206937-RNA, Messenger,
pubmed-meshheading:17206937-Recombinant Proteins
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT.
|
|
pubmed:affiliation |
Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, 715 Albany Street W509, Boston, MA 02118, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
