Linked Life Data

1720622

Source:http://linkedlifedata.com/resource/pubmed/id/1720622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1992-1-16
pubmed:databankReference
pubmed:abstractText
Polymerase chain reactions (PCR) are used to generate specific DNA sequences from minute amounts of DNA templates using a pair of oligonucleotide primers. To amplify regions of unknown sequence, methods such as inverted PCR, Alu PCR, and rapid amplification of cDNA ends (RACE) have been developed. These methods require several enzymatic manipulations of DNA which are either tedious or only suitable for certain special conditions. We have explored the possibility of PCR using a single primer. This method takes advantage of the fact that partial complementarity provides sufficient affinity for the oligonucleotide primer to anneal to a secondary, imperfect binding site. Thus, no modification of DNA template was required for the single primer-mediated PCR. We have used this method to generate two different aFGF cDNA clones containing different 5'-untranslated sequences.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
771-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Single primer-mediated polymerase chain reaction: application in cloning of two different 5'-untranslated sequences of acidic fibroblast growth factor mRNA.
pubmed:affiliation
Department of Internal Medicine, Ohio State University, Columbus 43210.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't