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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-1-8
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pubmed:abstractText |
The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the transcriptional level by the availability of iron. The PCR-amplified gene was cloned into the expression vector pTrc 99b and expressed in Escherichia coli. High concentrations of flavodoxin were found (20% of total protein). The recombinant protein was purified from the cytosolic fraction of the cells and it exhibited properties identical with those of the wild-type Anabaena flavodoxin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-14284756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-14907713,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-2119231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-2202597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-2204423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-2448875,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-2500643,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1720613-6406674
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
280 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-91
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:1720613-Amino Acid Sequence,
pubmed-meshheading:1720613-Anabaena,
pubmed-meshheading:1720613-Base Sequence,
pubmed-meshheading:1720613-Blotting, Northern,
pubmed-meshheading:1720613-Blotting, Southern,
pubmed-meshheading:1720613-Cloning, Molecular,
pubmed-meshheading:1720613-DNA, Bacterial,
pubmed-meshheading:1720613-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1720613-Escherichia coli,
pubmed-meshheading:1720613-Flavodoxin,
pubmed-meshheading:1720613-Gene Expression,
pubmed-meshheading:1720613-Genes, Bacterial,
pubmed-meshheading:1720613-Molecular Sequence Data,
pubmed-meshheading:1720613-Molecular Weight,
pubmed-meshheading:1720613-Polymerase Chain Reaction,
pubmed-meshheading:1720613-RNA, Bacterial,
pubmed-meshheading:1720613-Recombinant Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119.
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pubmed:affiliation |
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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