Source:http://linkedlifedata.com/resource/pubmed/id/17205186
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2007-1-5
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| pubmed:abstractText |
Regeneration of severed limbs in adult animals is restricted to urodele amphibians. Mammals, including humans, have very limited regenerative capabilities and even with proper treatment, only the tips of our digits can grow back. Teleost fish can regenerate amputated fins, the evolutionary ancestors of limbs. To elucidate the principles of limb-fin regeneration, we performed an Affymetrix microarray screen on regenerating caudal fins 12, 24, 48, and 72 h post amputation. Approximately 15,000 zebrafish transcripts were analyzed, identifying 829 transcripts as differentially expressed during regeneration. Of those, 563 were up-regulated and 266 were down-regulated. We constructed a comprehensive database containing expression data, functional assignment, and background information from the literature for each differentially expressed transcript. In order to validate our findings, we employed three approaches: (1) microarray expression analysis of genes previously implicated in fin regeneration, (2) RT-PCR analysis of genes newly identified as differentially expressed during regeneration, and (3) in situ hybridization of the up-regulated genes bambi, dlx5A, and her6. Moreover, we show that Smad 1/5/8 proteins, effector molecules of Bmp signaling, are phosphorylated during fin regeneration. Taken together, we provide a comprehensive database of fin regeneration that will serve as an important tool for understanding the molecular mechanisms of regeneration.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/Her6 protein, zebrafish,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Smad Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/dlx5a protein, zebrafish
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1537-744X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
6 Suppl 1
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
38-54
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| pubmed:dateRevised |
2007-12-3
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| pubmed:meshHeading |
pubmed-meshheading:17205186-Animals,
pubmed-meshheading:17205186-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:17205186-Databases, Genetic,
pubmed-meshheading:17205186-Epidermis,
pubmed-meshheading:17205186-Female,
pubmed-meshheading:17205186-Gene Expression Profiling,
pubmed-meshheading:17205186-Gene Expression Regulation,
pubmed-meshheading:17205186-Homeodomain Proteins,
pubmed-meshheading:17205186-Male,
pubmed-meshheading:17205186-Mesoderm,
pubmed-meshheading:17205186-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17205186-Regeneration,
pubmed-meshheading:17205186-Reproducibility of Results,
pubmed-meshheading:17205186-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17205186-Signal Transduction,
pubmed-meshheading:17205186-Smad Proteins,
pubmed-meshheading:17205186-Tail,
pubmed-meshheading:17205186-Transcription Factors,
pubmed-meshheading:17205186-Up-Regulation,
pubmed-meshheading:17205186-Wound Healing,
pubmed-meshheading:17205186-Zebrafish,
pubmed-meshheading:17205186-Zebrafish Proteins
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| pubmed:year |
2006
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| pubmed:articleTitle |
Transcriptional profiling of caudal fin regeneration in zebrafish.
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| pubmed:affiliation |
Howard Hughes Medical Institute, Department of Cardiology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA. mschebesta@enders.tch.harvard.edu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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