Beclin 1 is is an autophagy gene, the role of which as a tumour suppressor has recently been recognized in a few studies. We examined the expression of Beclin 1 protein in 212 primary human brain tumours, including 97 high-grade glial tumours, 29 low-grade glial tumours, 4 grade III meningiomas, 19 grade II meningiomas, 52 grade I meningiomas, and 11 medulloblastomas. In 94 cases, including 56 glial tumours, 35 meningiomas, and 3 medulloblastomas we also assessed Beclin 1 mRNA expression by real-time RT-PCR. In most high-grade astrocytic, ependymal neoplasms and atypical meningiomas we found a decrease of cytoplasmic protein expression that was, instead, high in the majority of low-grade tumours and in medulloblastomas. The expression level of Beclin 1 mRNA was significantly lower in glioblastomas than in grade II (p=0.04) and grade I (p=0.01) astrocytomas; in grade III than in grade I astrocytomas (p=0.01); in grade II than in grade I meningiomas (p=0.03); and in all glial tumours when compared to all meningiomas (p<0.0001). Cytoplasmic expression is thought to be linked to the functional protein. Our observations are in line with studies that demonstrated decreased expression of Beclin 1 in human breast, ovarian, prostate and ovarian cancer and furtherly support its involvement also in brain tumours, which had previously been demonstrated in a few experimental studies, both in spontaneous and in therapy-induced autophagy. Furthermore, our study suggests possible differences of Beclin 1 involvement and its role among the different histotypes of brain neoplasms. Further studies are needed to highlight Beclin 1 function in tumour suppression and/or in tumour survival through autophagy and other related programmed cell death processes in brain tumours.
pubmed-author:CosciElenaE, pubmed-author:De NisiMaria CaterinaMC, pubmed-author:FalzaranoSara MSM, pubmed-author:LuziPietroP, pubmed-author:MalagninoValeriaV, pubmed-author:MannucciSusannaS, pubmed-author:MiraccoCleliaC, pubmed-author:MonciattiIreneI, pubmed-author:OliveriGiuseppeG, pubmed-author:PacentiLorenzoL, pubmed-author:PirtoliLuigiL, pubmed-author:ToscanoMarziaM, pubmed-author:TosiPieroP
pubmed-meshheading:17203225-Apoptosis Regulatory Proteins, pubmed-meshheading:17203225-Autophagy, pubmed-meshheading:17203225-Brain Neoplasms, pubmed-meshheading:17203225-Cell Nucleus, pubmed-meshheading:17203225-Cytoplasm, pubmed-meshheading:17203225-Gene Expression Profiling, pubmed-meshheading:17203225-Gene Expression Regulation, Neoplastic, pubmed-meshheading:17203225-Humans, pubmed-meshheading:17203225-Immunohistochemistry, pubmed-meshheading:17203225-Medulloblastoma, pubmed-meshheading:17203225-Membrane Proteins, pubmed-meshheading:17203225-Meningioma, pubmed-meshheading:17203225-Oligodendroglioma, pubmed-meshheading:17203225-RNA, Messenger, pubmed-meshheading:17203225-Reverse Transcriptase Polymerase Chain Reaction
Protein and mRNA expression of autophagy gene Beclin 1 in human brain tumours.
Department of Human Pathology and Oncology, Section of Pathological Anatomy, University of Siena, I-53100 Siena, Italy. firstname.lastname@example.org