Linked Life Data

17203221

Source:http://linkedlifedata.com/resource/pubmed/id/17203221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-4
pubmed:abstractText
The aim of the present study was to clarify whether ATP binding cassette transporters are refractory factors in head and neck cancers. For in vitro and in vivo chemotherapeutic studies, we used the following head and neck cancer cell lines: a mouse oral squamous cell carcinoma (SCC) cell line, Sq-1979; a human SCC cell line, SCCHA; a mouse salivary gland adenocarcinoma (SGA) cell line, NR-PG; and a human SGA cell line, HSY. We used a vinca alkaloid anticancer drug, vincristine (VCR), as a chemotherapeutic anticancer drug. To determine the cause of multidrug resistance, Western blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry of xenografted tumors in nude mice, drug efflux analysis, and drug efflux inhibitory assays were performed. VCR-treated cell lines, Sq-1979/VCR, SCCHA/VCR, NR-PG/VCR, and HSY/VCR, intensively expressed multidrug resistance (MDR) gene 1 mRNA and multidrug resistance associated protein (MRP) 1 mRNA. MRP7 mRNA and protein were expressed in NR-PG/VCR and HSY/VCR cells, but not in Sq-1979/VCR and SCCHA/VCR cells. In each cell clone of NR-PG/VCR and HSY/VCR, MRP7 mRNA was induced by VCR treatment, suggesting an acquired resistance to VCR in the context of MRP7 expression. In the in vivo chemotherapeutic nude mice model, VCR-treated xenografted SCCHA and HSY cells expressed MDR1 and MRP1. Moreover, MRP7 expression was immunohistochemically found in xenografted HSY cells of VCR-injected tumor-bearing mice, but not in SCCHA cells. Furthermore, doxorubicin accumulation was increased and drug cross-resistance to docetaxel decreased in HSY/VCR in the presence of a competitive MRP7 inhibitor, 17-beta-estradiol-(17-beta-D-glucuronide). These results indicate that MDR1 expression, MRP1 expression, and MRP7 expression are refractory factors in head and neck cancer chemotherapy and suggest that induction of MRP7 expression is involved in drug resistance to natural products, especially to docetaxel in SGA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-401
pubmed:meshHeading
pubmed-meshheading:17203221-Adenocarcinoma, pubmed-meshheading:17203221-Animals, pubmed-meshheading:17203221-Antineoplastic Agents, pubmed-meshheading:17203221-Cell Line, Tumor, pubmed-meshheading:17203221-Drug Resistance, Multiple, pubmed-meshheading:17203221-Estradiol, pubmed-meshheading:17203221-Female, pubmed-meshheading:17203221-Gene Expression Regulation, Neoplastic, pubmed-meshheading:17203221-Head and Neck Neoplasms, pubmed-meshheading:17203221-Humans, pubmed-meshheading:17203221-Immunohistochemistry, pubmed-meshheading:17203221-Mice, pubmed-meshheading:17203221-Mice, Nude, pubmed-meshheading:17203221-Multidrug Resistance-Associated Proteins, pubmed-meshheading:17203221-Neoplasm Transplantation, pubmed-meshheading:17203221-RNA, Messenger, pubmed-meshheading:17203221-Salivary Gland Neoplasms, pubmed-meshheading:17203221-Taxoids
pubmed:year
2007
pubmed:articleTitle
Multidrug resistance-associated protein 7 expression is involved in cross-resistance to docetaxel in salivary gland adenocarcinoma cell lines.
pubmed:affiliation
Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry, Nagano 399-0781, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't