Linked Life Data

17202358

Source:http://linkedlifedata.com/resource/pubmed/id/17202358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-4
pubmed:abstractText
Ligands of the NKG2D receptor, which activates NK cells and costimulates effector T cells, are inducibly expressed under harmful conditions, such as malignancies and microbial infections. Moreover, aberrant expression in autoimmune disease lesions may contribute to disease progression. Among these ligands are the closely related human MHC class I-related chains (MIC) A and B, which appear to be regulated by cellular stress. Analyses of MIC gene 5'-end flanking regions in epithelial tumor cells defined minimal core promoters that directed near maximum heat shock- or oxidative stress-induced transcriptional activation. Considerably larger fully functional promoters were required for maximum proliferation-associated activation. These activities were dependent on core promoter sequences that included heat shock elements, which inducibly bound heat shock factor 1, TATA-like elements, and constitutively occupied Sp1 and inverted CCAAT box factor sites. By contrast, MIC gene activation by CMV infection was largely independent of these and upstream promoter sequences, and expression of viral immediate early gene (IE1 or IE2) products was sufficient for induction of transcription and surface protein expression. Altogether, these results reveal distinct modes of activation of the genes for the MIC ligands of NKG2D and provide a molecular framework for analyses of gene regulation under different cellular insult conditions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
961-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17202358-5' Flanking Region, pubmed-meshheading:17202358-Cell Line, Tumor, pubmed-meshheading:17202358-Gene Expression Regulation, pubmed-meshheading:17202358-Genes, Reporter, pubmed-meshheading:17202358-Heat-Shock Response, pubmed-meshheading:17202358-Histocompatibility Antigens Class I, pubmed-meshheading:17202358-Humans, pubmed-meshheading:17202358-Ligands, pubmed-meshheading:17202358-Molecular Sequence Data, pubmed-meshheading:17202358-Mutation, pubmed-meshheading:17202358-NK Cell Lectin-Like Receptor Subfamily K, pubmed-meshheading:17202358-Promoter Regions, Genetic, pubmed-meshheading:17202358-Protein Binding, pubmed-meshheading:17202358-Receptors, Immunologic, pubmed-meshheading:17202358-Receptors, Natural Killer Cell, pubmed-meshheading:17202358-Transcription, Genetic, pubmed-meshheading:17202358-Transcriptional Activation
pubmed:year
2007
pubmed:articleTitle
Promoter region architecture and transcriptional regulation of the genes for the MHC class I-related chain A and B ligands of NKG2D.
pubmed:affiliation
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural