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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-2-26
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pubmed:abstractText |
To determine the initiation strategy of the hepatitis E virus (HEV) open reading frame 3 (ORF3), we constructed five HEV mutants with desired mutations in the ORF1 and ORF2 junction region and tested their levels of in vivo infectivity in pigs. A mutant with a C-terminally truncated ORF3 is noninfectious in pigs, indicating that an intact ORF3 is required for in vivo infectivity. Mutations with substitutions in the first in-frame AUG in the junction region or with the same T insertion at the corresponding position of HEV genotype 4 did not affect the virus infectivity or rescue, although mutations with combinations of the two affected virus recovery efficiency, and a single mutation at the third in-frame AUG completely abolished virus infectivity in vivo, indicating that the third in-frame AUG in the junction region is required for virus infection and is likely the authentic initiation site for ORF3. A conserved double stem-loop RNA structure, which may be important for HEV replication, was identified in the junction region. This represents the first report of using a unique homologous pig model system to study the molecular mechanism of HEV replication and to systematically and definitively identify the authentic ORF3 initiation site.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-10859372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11097496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11160756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11230404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11518702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11536234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11562538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11773103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11884543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-11934888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-12655086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-12907011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-12917455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-12934945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-1335743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-15037615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-15166445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-15650181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-15731237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-15890906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-16099918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-16140784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-16172842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-16731930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-16928762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-1926770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-8980488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-9275216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17202216-9811705
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3018-26
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17202216-Animals,
pubmed-meshheading:17202216-Base Sequence,
pubmed-meshheading:17202216-Codon,
pubmed-meshheading:17202216-Hepatitis E virus,
pubmed-meshheading:17202216-Molecular Sequence Data,
pubmed-meshheading:17202216-Mutation,
pubmed-meshheading:17202216-Nucleic Acid Conformation,
pubmed-meshheading:17202216-Open Reading Frames,
pubmed-meshheading:17202216-RNA, Viral,
pubmed-meshheading:17202216-Sequence Homology, Nucleic Acid,
pubmed-meshheading:17202216-Swine,
pubmed-meshheading:17202216-Transcription Initiation Site,
pubmed-meshheading:17202216-Virulence
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pubmed:year |
2007
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pubmed:articleTitle |
Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo.
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pubmed:affiliation |
Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, 1410 Price's Fork Road, Blacksburg, VA 24601, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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