Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-1-2
pubmed:abstractText
Chromatin-induced spindle assembly depends on regulation of microtubule-depolymerizing proteins by the chromosomal passenger complex (CPC), consisting of Incenp, Survivin, Dasra (Borealin), and the kinase Aurora B, but the mechanism and significance of the spatial regulation of Aurora B activity remain unclear. Here, we show that the Aurora B pathway is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activated by chromatin via a Ran-independent mechanism. While spindle microtubule assembly normally requires Dasra-dependent chromatin binding of the CPC, this function of Dasra can be bypassed by clustering Aurora B-Incenp by using anti-Incenp antibodies, which stimulate autoactivation among bound complexes. However, such chromatin-independent Aurora B pathway activation promotes centrosomal microtubule assembly and produces aberrant achromosomal spindle-like structures. We propose that chromosomal enrichment of the CPC results in local kinase autoactivation, a mechanism that contributes to the spatial regulation of spindle assembly and possibly to other mitotic processes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-10408446, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-10966104, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-10970807, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11139336, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11163242, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11163243, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11179426, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11229403, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11285281, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11350965, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11375490, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11724818, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-11792540, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12048181, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12221116, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12535669, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12620232, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12707312, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12783991, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12805209, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-12925766, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-14521840, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-14530258, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-14722118, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-14960279, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-14972678, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15031504, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15064354, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15249581, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15260989, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15283590, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15351889, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15525506, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15616188, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15851029, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-15866179, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16083426, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16123300, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16239925, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16332542, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16351129, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16478663, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16537398, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16571672, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16572176, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16777603, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-16950107, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-1999463, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-2155777, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-2566917, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-9335509, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-9891325, http://linkedlifedata.com/resource/pubmed/commentcorrection/17199039-9989498
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts, http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone, http://linkedlifedata.com/resource/pubmed/chemical/Dasra A protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/DasraB protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/Histones, http://linkedlifedata.com/resource/pubmed/chemical/INCENP protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Stathmin, http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins, http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase, http://linkedlifedata.com/resource/pubmed/chemical/ran GTP-Binding Protein
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1534-5807
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-43
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