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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-2-5
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pubmed:abstractText |
NF-kappaB is an inducible transcription factor activated in many different cell types by inflammatory and stress signals. The transcription of a wide variety of NF-kappaB genes is regulated by the coordinated action of transcription co-activators and co-repressors. Previously we identified Myb binding protein 1a (MYBBP1a) as an interaction partner of the transcription activation domain of RelA/p65. MYBBP1a has been shown by others to regulate various transcription factors, through largely unknown mechanisms. Here we present evidence that MYBBP1a is a novel co-repressor of NF-kappaB. MYBBP1a interacted directly with RelA/p65 and expression of MYBBP1a in cells repressed NF-kappaB dependent reporter expression but did affect neither RelA/p65 nuclear translocation nor its DNA binding activity. In vitro, MYBBP1a inhibited transcription from chromatinized templates at a step before pre-initiation complex formation. MYBBP1a was found to compete with the histone acetyl transferase co-activator, p300, for interaction with the transcription activation domain of RelA/p65. Expression levels of MYBBP1a are dependent on the cell type, and are particularly high in Jurkat T cells. These results indicate that MYBBP1a is a novel NF-kappaB co-repressor of transcription that competes with p300 and may function to regulate cell type specific genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
366
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
725-36
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17196614-Active Transport, Cell Nucleus,
pubmed-meshheading:17196614-Binding, Competitive,
pubmed-meshheading:17196614-Cell Nucleolus,
pubmed-meshheading:17196614-DNA,
pubmed-meshheading:17196614-Genes, Reporter,
pubmed-meshheading:17196614-HeLa Cells,
pubmed-meshheading:17196614-Humans,
pubmed-meshheading:17196614-Jurkat Cells,
pubmed-meshheading:17196614-Nuclear Proteins,
pubmed-meshheading:17196614-Nucleocytoplasmic Transport Proteins,
pubmed-meshheading:17196614-Nucleosomes,
pubmed-meshheading:17196614-Protein Binding,
pubmed-meshheading:17196614-Protein Structure, Tertiary,
pubmed-meshheading:17196614-Protein Transport,
pubmed-meshheading:17196614-Repressor Proteins,
pubmed-meshheading:17196614-Transcription, Genetic,
pubmed-meshheading:17196614-Transcription Factor RelA,
pubmed-meshheading:17196614-Transcriptional Activation,
pubmed-meshheading:17196614-p300-CBP Transcription Factors
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pubmed:year |
2007
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pubmed:articleTitle |
MYBBP1a is a novel repressor of NF-kappaB.
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pubmed:affiliation |
Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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