Linked Life Data

17193708

Source:http://linkedlifedata.com/resource/pubmed/id/17193708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-12-27
pubmed:abstractText
An in vitro assay system was developed for accurate transcription of the octopine type T-DNA gene in a wheat germ extract. The system consists of the protein fraction extracted from the chromatin of wheat germ, substrates and exogenously added DNA. Specific initiation at the promoter was determined by a combination of primer extension analysis and size analysis of the transcripts synthesized from DNA templates of various molecular sizes. Synthesis of the transcripts was sensitive to alpha-amanitin. With truncated DNA templates containing the intact promoter and the proximal transcribing region of several hundred base pairs of the T-DNA, run-off transcripts of the expected size originating at the authentic promoter were synthesized together with relatively small amounts of prematurely terminated RNA molecules. On fractionation of the chromatin protein extract by DEAE-cellulose column chromatography, the fraction eluted with 0.3 M KCl showed no activity by itself for specific initiation of transcription at the promoter by RNA polymerase II. The activity was however restored by adding the fraction eluted with 0.15 M KCl, and the reconstituted RNA polymerase fraction correctly initiated transcription at the authentic promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
209
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
445-52
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Selective and accurate initiation of transcription at the T-DNA promoter in a soluble chromatin extract from wheat germ.
pubmed:affiliation
Laboratory of Molecular Genetics, Tsukuba Life Science Center, Riken 3-1-1 Koyadai, Yatabe-machi, Tsukuba-gun, Ibaraki, 305 Japan.
pubmed:publicationType
Journal Article