Linked Life Data

17193169

Source:http://linkedlifedata.com/resource/pubmed/id/17193169

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8-9
pubmed:dateCreated
2006-12-28
pubmed:abstractText
A new light-scattering-based method to detect molecular interactions at the surface of low-refractive-index nanoparticles was recently proposed. Water-dispersed nanoparticles functionalized with receptors typical of immature bacteria cell walls were used to study the activity of the antibiotic vancomycin. This method subtly depends on the specific properties of the nanoparticles. Here we discuss, by comparative experiments and through theoretical evaluation, the effects of size, refractive index, electric charge, and dilution on the reliability and accuracy of the method. Quite surprisingly, perfect index matching and minimal size (i.e., maximum surface), which is almost attained in one of the colloids here employed, do not represent the ideal conditions. Rather, we show that a nanoparticle radius of 100 nm and a refractive index slightly below that of water yields the best signal/background amplitude. We also show that repulsive interactions can lead to artifacts in the adsorption isotherm, thus indicating that electrostatic stabilization should be kept at a minimum. The close agreement between the interaction strengths, as measured with two different nanoparticle systems, testifies to the reliability of the method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1613-6829
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1060-7
pubmed:dateRevised
2008-4-17
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Phantom nanoparticles as probes of biomolecular interactions.
pubmed:affiliation
Istituto di Scienze e Tecnologie Molecolari-CNR via Golgi 19, 20133 Milano, Italy. davide.prosperi@unimi.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't