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rdf:type |
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lifeskim:mentions |
umls-concept:C0002726,
umls-concept:C0004561,
umls-concept:C0005768,
umls-concept:C0005953,
umls-concept:C0009013,
umls-concept:C0021038,
umls-concept:C0032112,
umls-concept:C0205314,
umls-concept:C0679622,
umls-concept:C0680948,
umls-concept:C0936012,
umls-concept:C1522642,
umls-concept:C1704387
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pubmed:issue |
1
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pubmed:dateCreated |
2007-2-8
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|
pubmed:abstractText |
Immunoglobulin light chain amyloidosis (AL) is characterized by a limited clonal expansion of plasma cells and amyloid formation. Here, we report restriction in the diversity of VL gene usage with a dominance of clonally related B cells in the peripheral blood (PB) isotype-specific repertoire of AL patients. A rigorous quantification of lineage trees reveals presence of intraclonal variations in the PB clones compared to the bone marrow (BM) clones, which suggests a common precursor that is still subject to somatic mutation. When compared to normal BM and PB B cells, AL clones showed significant but incomplete impairment of antigenic selection, which could not be detected by conventional R and S mutation analysis. Therefore, graphical analysis of B cell lineage trees and mathematical quantification of tree properties provide novel insights into the process of B cell clonal evolution in AL.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0271-9142
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
69-87
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pubmed:meshHeading |
pubmed-meshheading:17192818-Algorithms,
pubmed-meshheading:17192818-Amyloidosis,
pubmed-meshheading:17192818-Bone Marrow Cells,
pubmed-meshheading:17192818-Clone Cells,
pubmed-meshheading:17192818-Female,
pubmed-meshheading:17192818-Flow Cytometry,
pubmed-meshheading:17192818-Genes, Immunoglobulin,
pubmed-meshheading:17192818-Humans,
pubmed-meshheading:17192818-Immunoglobulin Heavy Chains,
pubmed-meshheading:17192818-Immunoglobulin Light Chains,
pubmed-meshheading:17192818-Immunoglobulin Variable Region,
pubmed-meshheading:17192818-Immunophenotyping,
pubmed-meshheading:17192818-Male,
pubmed-meshheading:17192818-Models, Biological,
pubmed-meshheading:17192818-Molecular Sequence Data,
pubmed-meshheading:17192818-Multiple Myeloma,
pubmed-meshheading:17192818-Plasma Cells,
pubmed-meshheading:17192818-Sequence Alignment,
pubmed-meshheading:17192818-Somatic Hypermutation, Immunoglobulin
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pubmed:year |
2007
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pubmed:articleTitle |
Novel analysis of clonal diversification in blood B cell and bone marrow plasma cell clones in immunoglobulin light chain amyloidosis.
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pubmed:affiliation |
Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA. abraham.roshini@mayo.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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