Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-12-26
pubmed:abstractText
A recombinant virus from which the start codon and 53% of the UL20 open reading frame had been deleted was constructed and characterized. We report the following: (i) The UL20- mutant formed small plaques in 143 tk- cells but failed to form plaques in Vero cells. Virus yields were approximately 10- to 100-fold lower than those of wild-type virus in all cell lines tested. (ii) Electron microscopic examination of Vero cells infected with the UL20- mutant revealed that enveloped and unenveloped capsids accumulated in the cytoplasm, possibly in the space between the inner and outer lamellae of the nuclear membrane, and that virtually no virus was present in the extracellular space. (iii) Glycoproteins B, C, D, E, H, and I recovered from lysates of cells infected with the UL20- mutant could not be differentiated from those present in lysates of cells infected with the wild-type parent virus with respect to the electrophoretic mobility of mature and precursor forms. (iv) Repair of the deleted sequences restored the wild-type phenotype. (v) The gene product of the UL20 gene was shown to be associated with cellular membranes and to possess characteristics of integral membrane proteins. We conclude that the UL20 gene encodes an integral membrane protein with a hitherto unrecognized function in that it enables the transit of virions to the extracellular space. The function of the UL20 gene product is complemented by some cell lines but not by Vero cells. The vesicles which serve to transport virions may have an origin different from those associated with transport of normal cellular proteins.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-167196, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-169022, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-172900, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-176453, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1845885, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1846207, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1847476, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1848309, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-1849972, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-185791, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2154589, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2173780, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-219254, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2194670, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2404139, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2688704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2824844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2838568, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2839594, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-284385, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-2990101, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3006335, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3009870, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3016991, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3029967, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3033823, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-3035578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-4110104, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-4300104, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6253078, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6254015, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6260657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6266147, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6268303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6277788, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6292449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6296278, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6298448, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6300459, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6301148, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6314098, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6316656, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6322419, http://linkedlifedata.com/resource/pubmed/commentcorrection/1719228-6330988
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:geneSymbol
U<down>L</down>20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6414-24
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:1719228-Humans, pubmed-meshheading:1719228-Animals, pubmed-meshheading:1719228-Microscopy, Electron, pubmed-meshheading:1719228-Glycoproteins, pubmed-meshheading:1719228-Simplexvirus, pubmed-meshheading:1719228-Base Sequence, pubmed-meshheading:1719228-Epitopes, pubmed-meshheading:1719228-DNA, Viral, pubmed-meshheading:1719228-Amino Acid Sequence, pubmed-meshheading:1719228-Thymidine Kinase, pubmed-meshheading:1719228-Cytomegalovirus, pubmed-meshheading:1719228-Virus Replication, pubmed-meshheading:1719228-Cell Line, pubmed-meshheading:1719228-Viral Plaque Assay, pubmed-meshheading:1719228-Recombination, Genetic, pubmed-meshheading:1719228-Genes, Viral, pubmed-meshheading:1719228-Molecular Sequence Data, pubmed-meshheading:1719228-Chromosome Deletion, pubmed-meshheading:1719228-Plasmids, pubmed-meshheading:1719228-Oligodeoxyribonucleotides
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